Hydrazines as substrates and inhibitors of the archaeal ammonia oxidation pathway

Schatteman, Arne, Wright, Chloë L., Crombie, Andrew T., Murrell, J. Colin and Lehtovirta-Morley, Laura E. (2022) Hydrazines as substrates and inhibitors of the archaeal ammonia oxidation pathway. Applied and Environmental Microbiology, 88 (8). ISSN 0099-2240

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Abstract

Ammonia-oxidizing archaea (AOA) and bacteria (AOB) perform key steps in the global nitrogen cycle, the oxidation of ammonia to nitrite. While the ammonia oxidation pathway is well characterized in AOB, many knowledge gaps remain about the metabolism of AOA. Hydroxylamine is an intermediate in both AOB and AOA, but homologues of hydroxylamine dehydrogenase (HAO), catalyzing bacterial hydroxylamine oxidation, are absent in AOA. Hydrazine is a substrate for bacterial HAO, while phenylhydrazine is a suicide inhibitor of HAO. Here, we examine the effect of hydrazines in AOA to gain insights into the archaeal ammonia oxidation pathway. We show that hydrazine is both a substrate and an inhibitor for AOA and that phenylhydrazine irreversibly inhibits archaeal hydroxylamine oxidation. Both hydrazine and phenylhydrazine interfered with ammonia and hydroxylamine oxidation in AOA. Furthermore, the AOA “Candidatus Nitrosocosmicus franklandus” C13 oxidized hydrazine into dinitrogen (N2), coupling this reaction to ATP production and O2 uptake. This study expands the known substrates of AOA and suggests that despite differences in enzymology, the ammonia oxidation pathways of AOB and AOA are functionally surprisingly similar. These results demonstrate that hydrazines are valuable tools for studying the archaeal ammonia oxidation pathway.

Item Type:	Article
Additional Information:	Funding information: A.S. is funded by a Royal Society Dorothy Hodgkin fellowship enhancement award (RGF\EA\180300). C.L.W. is funded by a University of East Anglia-funded Ph.D. studentship. L.E.L.-M. is funded by a Royal Society Dorothy Hodgkin research fellowship (DH150187) and by a European Research Council (ERC) starting grant (UNITY 852993). J.C.M. received funding through the Earth and Life Systems Alliance, Norwich Research Park, and A.T.C. was supported by a Leverhulme Trust early-career fellowship (ECF-2016-626).
Uncontrolled Keywords:	sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Science > School of Environmental Sciences University of East Anglia Research Groups/Centres > Theme - ClimateUEA
UEA Research Groups:	Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Groups > Environmental Biology
Depositing User:	LivePure Connector
Date Deposited:	30 Nov 2024 01:45
Last Modified:	11 Dec 2024 01:42
URI:	https://ueaeprints.uea.ac.uk/id/eprint/97836
DOI:	10.1128/aem.02470-21

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