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ToolsAshford, Fiona, Best, Angus, Dunn, Steven J., Ahmed, Zahra, Siddiqui, Henna, Melville, Jordan, Wilkinson, Samuel, Mirza, Jeremy, Cumley, Nicola, Stockton, Joanne, Ferguson, Jack, Wheatley, Lucy, Ratcliffe, Elizabeth, Casey, Anna, Plant, Tim, Quick, Joshua, Richter, Alex, Loman, Nicholas and McNally, Alan and The COVID-19 Genomics UK (COG-UK) Consortium (2022) SARS-CoV-2 testing in the community: Testing positive samples with the TaqMan SARS-CoV-2 mutation panel to find variants in real time. Journal of Clinical Microbiology, 60 (4). ISSN 0095-1137
Full text not available from this repository.Abstract
Genome sequencing is a powerful tool for identifying SARS-CoV-2 variant lineages; however, there can be limitations due to sequence dropout when used to identify specific key mutations. Recently, ThermoFisher Scientific has developed genotyping assays to help bridge the gap between testing capacity and sequencing capability to generate real-time genotyping results based on specific variants. Over a 6-week period during the months of April and May 2021, we set out to assess the ThermoFisher TaqMan mutation panel genotyping assay, initially for three mutations of concern and then for an additional two mutations of concern, against SARS-CoV-2- positive clinical samples and the corresponding COVID-19 Genomics UK Consortium (COG-UK) sequencing data. We demonstrate that genotyping is a powerful in-depth technique for identifying specific mutations, is an excellent complement to genome sequencing, and has real clinical health value potential, allowing laboratories to report and take action on variants of concern much more quickly.
| Item Type: | Article |
|---|---|
| Additional Information: | All Rights Reserved. This article is made available via the PMC Open Access Subset for unrestricted noncommercial re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. Funding Information: The UoB Turnkey COVID-19 diagnostic lab was created wholly from funds directly attributed by DHSC. COG-UK is supported by funding from the Medical Research Council (MRC) part of UK Research &hx0026; Innovation (UKRI), the National Institute of Health Research (NIHR) (grant code: MC_PC_19027), and Genome Research Limited, operating as the Wellcome Sanger Institute. |
| Uncontrolled Keywords: | genome sequencing,genotyping,pcr,real time,sars-cov-2,snps,variants of concern,microbiology (medical) ,/dk/atira/pure/subjectarea/asjc/2700/2726 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging Faculty of Medicine and Health Sciences > Research Groups > Musculoskeletal Medicine Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 30 Nov 2024 01:45 |
| Last Modified: | 02 Dec 2024 01:44 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97835 |
| DOI: | 10.1128/jcm.02408-21 |
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