Genetic requirements for uropathogenic E. coli proliferation in the bladder cell infection cycle

Mediati, Daniel G., Blair, Tamika A., Costas, Ariana, Monahan, Leigh G., Söderström, Bill, Charles, Ian G. and Duggin, Iain G. (2024) Genetic requirements for uropathogenic E. coli proliferation in the bladder cell infection cycle. mSystems, 9 (10). ISSN 2379-5077

Preview

PDF (mediati-et-al-2024-genetic-requirements-for-uropathogenic-e-coli-proliferation-in-the-bladder-cell-infection-cycle) - Published Version Available under License Creative Commons Attribution. Download (2MB) | Preview

Abstract

Uropathogenic Escherichia coli (UPEC) requires an adaptable physiology to survive the wide range of environments experienced in the host, including gut and urinary tract surfaces. To identify UPEC genes required during intracellular infection, we developed a transposon-directed insertion-site sequencing approach for cellular infection models and searched for genes in a library of ~20,000 UTI89 transposon-insertion mutants that are specifically required at the distinct stages of infection of cultured bladder epithelial cells. Some of the bacterial functional requirements apparent in host bladder cell growth overlapped with those for M9-glycerol, notably nutrient utilization, polysaccharide and macromolecule precursor biosynthesis, and cell envelope stress tolerance. Two genes implicated in the intracellular bladder cell infection stage were confirmed through independent gene deletion studies: neuC (sialic acid capsule biosynthesis) and hisF (histidine biosynthesis). Distinct sets of UPEC genes were also implicated in bacterial dispersal, where UPEC erupts from bladder cells in highly filamentous or motile forms upon exposure to human urine, and during recovery from infection in a rich medium. We confirm that the dedD gene linked to septal peptidoglycan remodeling is required during UPEC dispersal from human bladder cells and may help stabilize cell division or the cell wall during envelope stress created by host cells. Our findings support a view that the host intracellular environment and infection cycle are multi-nutrient limited and create stress that demands an array of biosynthetic, cell envelope integrity, and biofilm-related functions of UPEC.

Item Type:	Article
Uncontrolled Keywords:	tradis,upec,uti,cystitis,intracellular infection,stage-resolved model,microbiology,physiology,biochemistry,ecology, evolution, behavior and systematics,modelling and simulation,molecular biology,genetics,computer science applications ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
Related URLs:	http://www.scopus.com/inward/record.url?... https://journals.asm.org/doi/10.1128/msy...
Depositing User:	LivePure Connector
Date Deposited:	04 Nov 2024 12:30
Last Modified:	12 Nov 2024 14:31
URI:	https://ueaeprints.uea.ac.uk/id/eprint/97455
DOI:	10.1128/msystems.00387-24

Downloads

Actions (login required)

View Item