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ToolsJones, Emily J., Stentz, Regis, Parker, Aimée and Carding, Simon R. (2024) Assessing In Vivo Bacterial Extracellular Vesicle (BEV) Biodistribution Using Fluorescent Lipophilic Membrane Stains. In: Bacterial Extracellular Vesicles: Methods and Protocols. Methods in Molecular Biology . Humana Press Inc, pp. 239-251. ISBN 978-1-0716-4057-9
Full text not available from this repository. (Request a copy)Abstract
Bacterial extracellular vesicles (BEVs) are nano-size vesicles containing a cargo of bioactive molecules that can play key roles in microbe-microbe and microbe-host interactions. In tracking their biodistribution in vivo, BEVs can cross several physical host barriers including the intestinal epithelium, vascular endothelium, and blood-brain-barrier (BBB) to ultimately accumulate in tissues such as the liver, lungs, spleen, and the brain. This tissue-specific dissemination has been exploited for the delivery of biomolecules such as vaccines for mucosal delivery. Although numerous strategies for labeling and tracking BEVs have been described, most have constraints that impact on interpreting in vivo bioimaging patterns. Here, we describe a general method for labeling BEVs using lipophilic fluorescent membrane stains which can be adopted by non-expert users. We also describe how the procedure can be used to overcome potential limitations. Furthermore, we outline methods of quantitative ex vivo tissue imaging that can be used to evaluate BEV organ trafficking.
| Item Type: | Book Section |
|---|---|
| Additional Information: | Funding Information: This work was supported by the Biological Sciences Research Council (BBSRC) Institute Strategic Programme Grant number BB/R012490/1 and its constituent projects BBS/E/F/ 000PR10353, BBS/E/F/000PR10355, and BBS/E/F/ 000PR10356. Publisher Copyright: © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024. |
| Uncontrolled Keywords: | bacterial extracellular vesicles (bevs),did,in vivo biodistribution,lipophilic,microbe-host,outer membrane vesicles (omvs),molecular biology,genetics,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/1300/1312 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 01 Nov 2024 17:30 |
| Last Modified: | 01 Nov 2024 17:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97446 |
| DOI: | 10.1007/978-1-0716-4055-5_15 |
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