Production, Isolation, and Characterization of Bioengineered Bacterial Extracellular Membrane Vesicles Derived from Bacteroides thetaiotaomicron and Their Use in Vaccine Development

Tools

Stentz, Régis, Miquel-Clopés, Ariadna and Carding, Simon R. (2021) Production, Isolation, and Characterization of Bioengineered Bacterial Extracellular Membrane Vesicles Derived from Bacteroides thetaiotaomicron and Their Use in Vaccine Development. In: Bacterial Vaccines: Methods and Protocols. Methods in Molecular Biology . Humana Press Inc, pp. 171-190. ISBN 978-1-0716-1902-5

Full text not available from this repository. (Request a copy)

Abstract

Bacterial extracellular vesicles (BEVs) possess features that make them well suited for the delivery of therapeutics and vaccines. This chapter describes methods for engineering the commensal human intestinal bacterium Bacteroides thetaiotaomicron (Bt) to produce BEVs carrying vaccine antigens and accompanying methods for isolating and purifying BEVs for mucosal vaccination regimens.

Item Type:	Book Section
Additional Information:	Funding Information: This work was supported in part by the UK Biotechnology and Biological Sciences Research Council (BBSRC) under grant numbers BB/J004529/1, BB/R012490/1, and BBS/E/ F000PR10355. We thank Dr. Emily Jones for her contribution in setting up methods for SEC purification and the use of the Zeta-view NTA, and Dr. Rokas Juodeikis for contributing to the development of BDM+ medium. Publisher Copyright: © 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Uncontrolled Keywords:	bacterial extracellular vesicles,bacteroides thetaiotaomicron,crossflow ultrafiltration,immunization,vaccines,molecular biology,genetics,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/1300/1312
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	01 Nov 2024 17:30
Last Modified:	01 Nov 2024 17:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/97445
DOI:	10.1007/978-1-0716-1900-1_11

Actions (login required)

View Item