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ToolsArora, Sanu, Steed, Andrew, Goddard, Rachel, Gaurav, Kumar, O’Hara, Tom, Schoen, Adam, Rawat, Nidhi, Elkot, Ahmed F., Korolev, Andrey V., Chinoy, Catherine, Nicholson, Martha H., Asuke, Soichiro, Antoniou-Kourounioti, Rea, Steuernagel, Burkhard, Yu, Guotai, Awal, Rajani, Forner-Martínez, Macarena, Wingen, Luzie, Baggs, Erin, Clarke, Jonathan, Saunders, Diane G.O., Krasileva, Ksenia V., Tosa, Yukio, Jones, Jonathan D.G., Tiwari, Vijay K., Wulff, Brande B.H. and Nicholson, Paul (2023) A wheat kinase and immune receptor form host-specificity barriers against the blast fungus. Nature Plants, 9 (3). pp. 385-392. ISSN 2055-026X
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Abstract
Since emerging in Brazil in 1985, wheat blast has spread throughout South America and recently appeared in Bangladesh and Zambia. Here we show that two wheat resistance genes, Rwt3 and Rwt4, acting as host-specificity barriers against non-Triticum blast pathotypes encode a nucleotide-binding leucine-rich repeat immune receptor and a tandem kinase, respectively. Molecular isolation of these genes will enable study of the molecular interaction between pathogen effector and host resistance genes.
| Item Type: | Article |
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| Additional Information: | Data availability: The RenSeq 150 bp paired-end Illumina sequences (raw data) for the 300 Watkins and 21 non-Watkins lines (including Anahuac) and the cDNA RenSeq data of 6 Watkins lines are available from NCBI study number PRJNA760793. The k-mer matrix and the CLC assemblies of the 300 Watkins and 21 non-Watkins lines are available from Zenodo under the following DOIs: https://doi.org/10.5281/zenodo.5557564, https://doi.org/10.5281/zenodo.5557685, https://doi.org/10.5281/zenodo.5557721, https://doi.org/10.5281/zenodo.5557827, https://doi.org/10.5281/zenodo.5557838 and https://doi.org/10.5281/zenodo.5655720. The genomic sequences of Rwt3 and Rwt4 are available from the wheat Ensembl databases as TraesCS1D02G029900 from https://plants.ensembl.org/Triticum_aestivum/ and TraesJAG1D03G00423590 from https://plants.ensembl.org/Triticum_aestivum_jagger/, respectively. Code availability: Scripts for the Watkins k-mer matrix generation, phylogenetic tree construction and k-mer-based association mapping can be found in the repository https://github.com/arorasanu/watkins_renseq. Funding Information: The high-performance computing resources and services used in this work were supported by the Norwich Bioscience Institutes Partnership (NBIP) Computing infrastructure for Science (CiS) group alongside the Earlham Institute (EI) scientific computing group. We are grateful to the John Innes Centre (JIC) Horticultural Services for plant husbandry; EI for providing open access to the Kronos genome; R. Goram at the JIC genotyping platform for the KASP genotyping. This research was financed by the Biotechnology and Biological Sciences Research Council (BBSRC) Designing Future Wheat Cross-Institute Strategic Programme to B.B.H.W. and P.N. (BBS/E/J/000PR9780); a John Innes Centre Institute Strategic Grant to B.B.H.W.; Science, Technology & Innovation Funding Authority (STDF), Egypt-UK Newton-Mosharafa Institutional Links award, Project ID (30718) to A.F.E. and B.B.H.W.; the Gordon and Betty Moore Foundation through grant GBMF4725 to the Two Blades Foundation; and the Gatsby Charitable Foundation to JDGJ; National Science Foundation (award number 1943155) and USDA NIFA (award numbers 2020-67013-32558 and 2020-67013-31460) to N.R. and V.K.T.; European Research Commission grant (ERC-2016-STG-716233-MIREDI) to K.V.K. and BBSRC Norwich Research Park Doctoral Training Grant (BB/M011216/1) for supporting E.B. Funding Information: The high-performance computing resources and services used in this work were supported by the Norwich Bioscience Institutes Partnership (NBIP) Computing infrastructure for Science (CiS) group alongside the Earlham Institute (EI) scientific computing group. We are grateful to the John Innes Centre (JIC) Horticultural Services for plant husbandry; EI for providing open access to the Kronos genome; R. Goram at the JIC genotyping platform for the KASP genotyping. This research was financed by the Biotechnology and Biological Sciences Research Council (BBSRC) Designing Future Wheat Cross-Institute Strategic Programme to B.B.H.W. and P.N. (BBS/E/J/000PR9780); a John Innes Centre Institute Strategic Grant to B.B.H.W.; Science, Technology & Innovation Funding Authority (STDF), Egypt-UK Newton-Mosharafa Institutional Links award, Project ID (30718) to A.F.E. and B.B.H.W.; the Gordon and Betty Moore Foundation through grant GBMF4725 to the Two Blades Foundation; and the Gatsby Charitable Foundation to JDGJ; National Science Foundation (award number 1943155) and USDA NIFA (award numbers 2020-67013-32558 and 2020-67013-31460) to N.R. and V.K.T.; European Research Commission grant (ERC-2016-STG-716233-MIREDI) to K.V.K. and BBSRC Norwich Research Park Doctoral Training Grant (BB/M011216/1) for supporting E.B. |
| Uncontrolled Keywords: | plant science ,/dk/atira/pure/subjectarea/asjc/1100/1110 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Plant Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 31 Oct 2024 17:30 |
| Last Modified: | 01 Nov 2024 11:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97401 |
| DOI: | 10.1038/s41477-023-01357-5 |
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