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Brabham, Helen J., De La Cruz, Diana Gómez, Were, Vincent, Shimizu, Motoki, Saitoh, Hiromasa, Hernández-Pinzón, Inmaculada, Green, Phon, Lorang, Jennifer, Fujisaki, Koki, Sato, Kazuhiro, Molnár, István, Šimková, Hana, Doležel, Jaroslav, Russell, James, Taylor, Jodie, Smoker, Matthew, Gupta, Yogesh Kumar, Wolpert, Tom, Talbot, Nicholas J. 
ORCID: https://orcid.org/0000-0001-6434-7757, Terauchi, Ryohei and Moscou, Matthew J. ORCID: https://orcid.org/0000-0003-2098-6818
(2024)
Barley MLA3 recognizes the host-specificity effector Pwl2 from Magnaporthe oryzae.
The Plant Cell, 36 (2).
pp. 447-470.
ISSN 1040-4651
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Abstract
Plant nucleotide-binding leucine-rich repeat (NLRs) immune receptors directly or indirectly recognize pathogen-secreted effector molecules to initiate plant defense. Recognition of multiple pathogens by a single NLR is rare and usually occurs via monitoring for changes to host proteins; few characterized NLRs have been shown to recognize multiple effectors. The barley (Hordeum vulgare) NLR gene Mildew locus a (Mla) has undergone functional diversification, and the proteins encoded by different Mla alleles recognize host-adapted isolates of barley powdery mildew (Blumeria graminis f. sp. hordei [Bgh]). Here, we show that Mla3 also confers resistance to the rice blast fungus Magnaporthe oryzae in a dosage-dependent manner. Using a forward genetic screen, we discovered that the recognized effector from M. oryzae is Pathogenicity toward Weeping Lovegrass 2 (Pwl2), a host range determinant factor that prevents M. oryzae from infecting weeping lovegrass (Eragrostis curvula). Mla3 has therefore convergently evolved the capacity to recognize effectors from diverse pathogens.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: Funding for this research includes United Kingdom Research and Innovation-Biotechnology and Biological Sciences Research Council Norwich Research Park Doctoral Training Partnership (grant no. BB/M011216/1 to JR) and Institute Strategic Programme (grant no. BB/P012574/1 to M.J.M. and BBS/E/J/000 PR9795 to M.J.M.), European Regional Development Fund (grant no. CZ.02.1.01/0.0/0.0/16_019/0000827 to I.M., J.D., and H.Š.), Perry Foundation (H.J.B.), Japan Society for the Promotion of Science 2018 Summer Programme (H.J.B.), Gatsby Charitable Foundation (M.J.M. and N.J.T.), and United States Department of Agriculture-Agricultural Research Service CRIS #5062-21220-025-000D (M.J.M.). |
| Uncontrolled Keywords: | plant science ,/dk/atira/pure/subjectarea/asjc/1100/1110 |
| Faculty \ School: | Faculty of Science > The Sainsbury Laboratory Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 31 Oct 2024 16:30 |
| Last Modified: | 17 Dec 2024 01:41 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97396 |
| DOI: | 10.1093/plcell/koad266 |
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