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Ravi, Anuradha, Troncoso-Rey, Perla, Ahn-Jarvis, Jennifer, Corbin, Kendall R., Harris, Suzanne, Harris, Hannah, Aydin, Alp, Kay, Gemma L., Le Viet, Thanh, Gilroy, Rachel, Pallen, Mark J. 
ORCID: https://orcid.org/0000-0003-1807-3657, Page, Andrew J., O'Grady, Justin and Warren, Frederick J.
(2022)
Hybrid metagenome assemblies link carbohydrate structure with function in the human gut microbiome.
Communications Biology, 5.
ISSN 2399-3642
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Abstract
Complex carbohydrates that escape small intestinal digestion, are broken down in the large intestine by enzymes encoded by the gut microbiome. This is a symbiotic relationship between microbes and host, resulting in metabolic products that influence host health and are exploited by other microbes. However, the role of carbohydrate structure in directing microbiota community composition and the succession of carbohydrate-degrading microbes, is not fully understood. In this study we evaluate species-level compositional variation within a single microbiome in response to six structurally distinct carbohydrates in a controlled model gut using hybrid metagenome assemblies. We identified 509 high-quality metagenome-assembled genomes (MAGs) belonging to ten bacterial classes and 28 bacterial families. Bacterial species identified as carrying genes encoding starch binding modules increased in abundance in response to starches. The use of hybrid metagenomics has allowed identification of several uncultured species with the functional potential to degrade starch substrates for future study.
| Item Type: | Article |
|---|---|
| Additional Information: | Data availability statement: Raw read data from the PromethION and NovaSeq sequencing runs can be accessed through the NCBI SRA project number PRJNA722408 and can be accessed at http://www.ncbi.nlm.nih.gov/bioproject/722408. GenBank accession numbers for individual MAG’s within this ProjectID can be found in Supplementary Data 26. Source data underlying Fig. 3b, c are provided in Supplementary Data 16. Funding Information: The authors gratefully acknowledge the support of the Biotechnology and Biological Sciences Research Council (BBSRC). This research was funded by: the BBSRC Institute Strategic Programme (ISP) Food Innovation and Health BB/R012512/1 and its constituent projects (BBS/E/F/000PR10343, BS/E/F/000PR10346); the BBSRC ISP Microbes in the Food Chain BB/R012504/1 and its constituent projects (BBS/E/F/000PR10348, BBS/E/F/000PR10349, BBS/E/F/000PR10352); and the BBSRC Core Capability Grant (BB/CCG1860/1). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. |
| Uncontrolled Keywords: | medicine (miscellaneous),biochemistry, genetics and molecular biology(all),agricultural and biological sciences(all) ,/dk/atira/pure/subjectarea/asjc/2700/2701 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 31 Oct 2024 15:30 |
| Last Modified: | 04 Nov 2024 00:55 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97386 |
| DOI: | 10.1038/s42003-022-03865-0 |
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