Tools
Tools
Oliveira-Garcia, Ely, Tamang, Tej Man, Park, Jungeun, Dalby, Melinda, Martin-Urdiroz, Magdalena, Herrero, Clara Rodriguez, Vu, An Hong, Park, Sunghun, Talbot, Nicholas J. 
ORCID: https://orcid.org/0000-0001-6434-7757 and Valent, Barbara
(2023)
Clathrin-mediated endocytosis facilitates the internalization of Magnaporthe oryzae effectors into rice cells.
Plant Cell, 35 (7).
pp. 2527-2551.
ISSN 1040-4651
Preview |
PDF (koad094)
- Published Version
Available under License Creative Commons Attribution. Download (2MB) | Preview |
Abstract
Fungi and oomycetes deliver effectors into living plant cells to suppress defenses and control plant processes needed for infection. Little is known about the mechanism by which these pathogens translocate effector proteins across the plasma membrane into the plant cytoplasm. The blast fungus Magnaporthe oryzae secretes cytoplasmic effectors into a specialized biotrophic interfacial complex (BIC) before translocation. Here, we show that cytoplasmic effectors within BICs are packaged into punctate membranous effector compartments that are occasionally observed in the host cytoplasm. Live cell imaging with fluorescently labeled proteins in rice (Oryza sativa) showed that these effector puncta colocalize with the plant plasma membrane and with CLATHRIN LIGHT CHAIN 1, a component of clathrin-mediated endocytosis (CME). Inhibiting CME using virus-induced gene silencing and chemical treatments resulted in cytoplasmic effectors in swollen BICs lacking effector puncta. By contrast, fluorescent marker colocalization, gene silencing, and chemical inhibitor studies failed to support a major role for clathrin-independent endocytosis in effector translocation. Effector localization patterns indicated that cytoplasmic effector translocation occurs underneath appressoria before invasive hyphal growth. Taken together, this study provides evidence that cytoplasmic effector translocation is mediated by CME in BICs and suggests a role for M. oryzae effectors in coopting plant endocytosis.
| Item Type: | Article |
|---|---|
| Additional Information: | Data availability statement: All study data are included in the article and/or supporting information. All strains and plasmids generated in this study are available from the authors upon request. Funding Information: We acknowledge and thank Rick Nelson (Noble Foundation, Ardmore, OK) for providing the VIGS vectors and for hosting E.O.-G. in his laboratory to learn the VIGS assay. We thank the Talbot laboratory for assistance and hospitality during a working visit for E.O.-G at the University of Exeter. We thank Hiromasa Saitoh (Iwate Biotechnology Research Center, Kitakami, Iwate, Japan) and Ryohei Terauchi (Iwate Biotechnology Research Center, Kitakami, Iwate, and Kyoto University, Japan) for sharing transgenic rice expressing the plant plasma membrane marker Lti6B:GFP. We acknowledge Dr. David H. Burk and the Louisiana State University Shared Instrumentation Facility, for the help with confocal microscopy and imaging processing. This project was supported by Agriculture and Food Research Initiative Competitive Grant no. #2017-67013-26525 from the USDA National Institute of Food and Agriculture Plant Biotic Interactions program. Additional support came from Louisiana State University Agricultural Center Hatch project #LAB94477 and from Louisiana Board of Regents grant #LEQSF(2022-24)RD-A-01. This is Contribution no. 22-053-J from the Kansas Agricultural Experiment Station. |
| Uncontrolled Keywords: | plant science ,/dk/atira/pure/subjectarea/asjc/1100/1110 |
| Faculty \ School: | Faculty of Science > The Sainsbury Laboratory |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 31 Oct 2024 13:30 |
| Last Modified: | 03 Nov 2024 07:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97379 |
| DOI: | 10.1093/plcell/koad094 |
Downloads
Downloads per month over past year
Actions (login required)
 |
View Item |