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Tang, Bozeng, Yan, Xia, Ryder, Lauren S., Bautista, Mark Jave A., Cruz-Mireles, Neftaly, Soanes, Darren M., Molinari, Camilla, Foster, Andrew J. and Talbot, Nicholas J. 
ORCID: https://orcid.org/0000-0001-6434-7757
(2023)
Rgs1 is a regulator of effector gene expression during plant infection by the rice blast fungus Magnaporthe oryzae.
Proceedings of the National Academy of Sciences of the United States of America, 120 (12).
ISSN 0027-8424
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Abstract
To cause rice blast disease, the filamentous fungus Magnaporthe oryzae secretes a battery of effector proteins into host plant tissue to facilitate infection. Effectorencoding genes are expressed only during plant infection and show very low expression during other developmental stages. How effector gene expression is regulated in such a precise manner during invasive growth by M. oryzae is not known. Here, we report a forward-genetic screen to identify regulators of effector gene expression, based on the selection of mutants that show constitutive effector gene expression. Using this simple screen, we identify Rgs1, a regulator of G-protein signaling (RGS) protein that is necessary for appressorium development, as a novel transcriptional regulator of effector gene expression, which acts prior to plant infection. We show that an N-terminal domain of Rgs1, possessing transactivation activity, is required for effector gene regulation and acts in an RGS-independent manner. Rgs1 controls the expression of at least 60 temporally coregulated effector genes, preventing their transcription during the prepenetration stage of development prior to plant infection. A regulator of appressorium morphogenesis is therefore also required for the orchestration of pathogen gene expression required for invasive growth by M. oryzae during plant infection.
| Item Type: | Article |
|---|---|
| Additional Information: | Data, Materials, and Software Availability: RNA-seq data of M. oryzae and the genome sequence of cer7 mutant strain reported in this paper were deposited in the European Nucleotide Archive - EMBL-EBI database under accession number: PRJEB45710 (see ref 54 in paper). Funding Information: This project was supported by a European Research Council Advanced Investigator award (to N.J.T.) under the European Union’s Seventh Framework Programme FP7/2007-2013/ERC Grant Agreement 294702 GENBLAST, a grant to N.J.T. from The Gatsby Charitable Foundation, and a Halpin Scholarship to B.T. |
| Uncontrolled Keywords: | effectors,gene expression,plant pathogen,rice blast |
| Faculty \ School: | Faculty of Science > The Sainsbury Laboratory Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 31 Oct 2024 12:30 |
| Last Modified: | 17 Dec 2024 01:41 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97375 |
| DOI: | 10.1073/pnas.2301358120 |
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