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ToolsStoakes, Emily, Savva, George M., Coates, Ruby, Tejera, Noemi, Poolman, Mark G., Grant, Andrew J., Wain, John and Singh, Dipali (2022) Substrate utilisation and energy metabolism in non-growing Campylobacter jejuni M1cam. Microorganisms, 10 (7). ISSN 2076-2607
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Abstract
Campylobacter jejuni, the major cause of bacterial foodborne illness, is also a fastidious organism that requires strict growth requirements in the laboratory. Our aim was to study substrate utilisation and energy metabolism in non-growing C. jejuni to investigate the ability of these bacteria to survive so effectively in the food chain. We integrated phenotypic microarrays and genome-scale metabolic modelling (GSM) to investigate the survival of C. jejuni on 95 substrates. We further investigated the underlying metabolic re-adjustment associated with varying energy demands on each substrate. We identified amino acids, organic acids and H2, as single substrates supporting survival without growth. We identified several different mechanisms, which were used alone or in combination, for ATP production: substrate-level phosphorylation via acetate kinase, the TCA cycle, and oxidative phosphorylation via the electron transport chain that utilised alternative electron donors and acceptors. The benefit of ATP production through each of these mechanisms was associated with the cost of enzyme investment, nutrient availability and/or O2 utilisation. C. jejuni can utilise a wide range of substrates as energy sources, including organic acids commonly used for marination or preservation of ingredients, which might contribute to the success of their survival in changing environments.
| Item Type: | Article |
|---|---|
| Additional Information: | Data Availability Statement: The data that support the findings of this study are available, on request, from https://gitlab.com/singhdi/campy-biolog (accessed on 29 June 2022). The updated GSM has also been deposited to the BioModels repository https://www.ebi.ac.uk/biomodels/MODEL2207010001 (accessed on 29 June 2022). Funding Information: Funding: This research was funded by the UK Biotechnology and Biological Sciences Research Council (BBSRC), through the Institute Strategic Research Programme (ISP) BB/R012504/1 Microbes in the Food Chain and its constituent project Microbial Survival in the Food Chain BBS/E/F/000PR10349 and the BBSRC Core Capability Grant BB/CCG1860/1. |
| Uncontrolled Keywords: | campylobacter jejuni,cell survival,energy metabolism,metabolic network,substrate utilisation,microbiology,microbiology (medical),virology ,/dk/atira/pure/subjectarea/asjc/2400/2404 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > School of Health Sciences Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Science > Research Groups > Norwich Epidemiology Centre Faculty of Medicine and Health Sciences > Research Groups > Norwich Epidemiology Centre |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 31 Oct 2024 12:30 |
| Last Modified: | 31 Oct 2024 13:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97371 |
| DOI: | 10.3390/microorganisms10071355 |
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