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ToolsGrech-Baran, Marta, Witek, Kamil, Poznański, Jarosław T., Grupa-Urbańska, Anna, Malinowski, Tadeusz, Lichocka, Małgorzata, Jones, Jonathan D.G. and Hennig, Jacek (2022) The Rysto immune receptor recognises a broadly conserved feature of potyviral coat proteins. New Phytologist, 235 (3). pp. 1179-1195. ISSN 0028-646X
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Abstract
Knowledge of the immune mechanisms responsible for viral recognition is critical for understanding durable disease resistance and successful crop protection. We determined how potato virus Y (PVY) coat protein (CP) is recognised by Rysto, a TNL immune receptor. We applied structural modelling, site-directed mutagenesis, transient overexpression, co-immunoprecipitation, infection assays and physiological cell death marker measurements to investigate the mechanism of Rysto–CP interaction. Rysto associates directly with PVY CP in planta that is conditioned by the presence of a CP central 149 amino acids domain. Each deletion that affects the CP core region impairs the ability of Rysto to trigger defence. Point mutations in the amino acid residues Ser125, Arg157, and Asp201 of the conserved RNA-binding pocket of potyviral CP reduce or abolish Rysto binding and Rysto-dependent responses, demonstrating that appropriate folding of the CP core is crucial for Rysto-mediated recognition. Rysto recognises the CPs of at least 10 crop-damaging viruses that share a similar core region. It confers immunity to plum pox virus and turnip mosaic virus in both Solanaceae and Brassicaceae systems, demonstrating potential utility in engineering virus resistance in various crops. Our findings shed new light on how R proteins detect different viruses by sensing conserved structural patterns.
| Item Type: | Article |
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| Additional Information: | Data availability statement: The data that support the findings presented in this publication are available from the corresponding authors upon reasonable request. Acknowledgements: We would like to thank our co‐workers, in particular Dr Magdalena Krzymowska and Dr Agnieszka I. Witek, for critical comments on the manuscript. Prof. Jane Parker and Dr Johannes Stuttmann for sharing seeds of , and various knockout plants. Prof. Juan Antonio Garcia Alvarez and Dr Véronique Decroocq for sharing PPV‐GFP infectious clones. Prof. James Carrington for sharing TuMV‐GFP infectious clone. We would like to acknowledge Agata Gorczyca for technical support. Research was supported by an OPUS 14 (2017/27/B/NZ9/01803) grant from the National Science Centre, Poland to JH. MG‐B was supported by a SONATA 15 (2019/35/D/NZ9/03565) grant from the National Science Centre, Poland. The authors declare no competing interests. |
| Uncontrolled Keywords: | cell death,coat protein structure,potato virus y,potyvirus resistance engineering,ry,physiology,plant science ,/dk/atira/pure/subjectarea/asjc/1300/1314 |
| Faculty \ School: | Faculty of Science > The Sainsbury Laboratory Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Plant Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 31 Oct 2024 11:30 |
| Last Modified: | 01 Nov 2024 13:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97367 |
| DOI: | 10.1111/nph.18183 |
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