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Bailey, Nicholas P., Shao, Yuxin, Du, Shaodua, Foster, Peter G., Fettweis, Jennifer, Hall, Neil 
ORCID: https://orcid.org/0000-0003-2808-0009, Wang, Zheng and Hirt, Robert P.
(2023)
Evolutionary conservation of Trichomonas-mycoplasma symbiosis across the host species barrier.
Frontiers in Microbiology, 14.
ISSN 1664-302X
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Abstract
Introduction: The protozoan parasite Trichomonas vaginalis is the most common cellular sexually transmitted disease in humans, and the closely related species Trichomonas gallinae is an avian parasite of ecological and economic importance. Phylogenetic evidence suggests T. vaginalis arose during bird to human transmission of a T. gallinae-like ancestor. Trichomonas vaginalis shares a strong clinical association with the independent sexually transmitted pathogen Metamycoplasma (formerly Mycoplasma) hominis, and the uncultured bacterium “Candidatus Malacoplasma (formerly Mycoplasma) girerdii,” with the latter association being an order of magnitude stronger. Both bacterial species have been shown to profoundly influence T. vaginalis growth, energy production and virulence-associated mechanisms. Methods: Evidence for a novel Malacoplasma sp. was discovered by in vivo Illumina metatranscriptomics sequencing of the T. gallinae-infected pigeon mouth. We leveraged published 16S rDNA profiling data from digestive tract of 12 healthy and 24 T. gallinae-infected pigeons to investigate association between the novel Malacoplasma sp. and T. gallinae. We utilised Illumina metagenomics sequencing targeted to pigeon oral and crop samples infected with the novel Malacoplasma sp. to generate its full-length genome sequence. Sequence similarity network analysis was used to compare annotated proteins from the novel Malacoplasma sp. with a range of other related species. Results: Here we present evidence for a novel Malacoplasma species, related to “Ca. M. girerdii,” that is strongly associated with T. gallinae in the upper digestive tract of domestic pigeons. Analysis of the genome sequence revealed gene features apparently specific to a Trichomonas-symbiotic Malacoplasma lineage. Discussion: These data support a model of long-term association between Trichomonas and Malacoplasma spp. that has been conserved across diversification of the Trichomonas lineage and the host species barrier from birds to human.
| Item Type: | Article |
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| Additional Information: | Data availability statement: All the sequencing data and corresponding assemblies can be accessed from the NCBI with the following accessions: BioProject PRJNA797924: Metagenome, China, SRA: SRR17641486. Assembled genome, China, biosample: SAMN25041167. Metagenome, UK, SRA: SRR18744026. Metatranscriptome, UK, SRA: SRR17643316 AND SRR17643315. BioProject PRJNA816903: assembled genome, UK: assembly accession: SAMN25134305. Additional data are available in the supplementary material. Funding Information: This work was supported by the Biotechnology and Bioscience Research Council Doctoral Training Partnership for Newcastle, Liverpool and Durham (grant number: BB/M011186/1 – NPB PhD student, RH supervisor). Work in China was funded by Innovation capacity building project of Beijing Academy of Agriculture and Forestry Science (KJCX 20200404), and Beijing municipal science and technology commission national modern agricultural science and technology city industry cultivation and achievement benefit project (Z171100001517003). NH is supported by the Core strategic Program of the Earlham Institute BB/CCG1720/1. Next-generation sequencing of the UK derived sample was delivered via the BBSRC National Capability in Genomics and Single Cell Analysis BB/CCG1720/1 at Earlham Institute. |
| Uncontrolled Keywords: | mycoplasma girerdii,mycoplasma trichamica,metagenomics,metatranscriptomics,new bacteria species,phylogenetics,trichomonas gallinae,trichomonas vaginalis,microbiology,microbiology (medical),sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2400/2404 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
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| Depositing User: | LivePure Connector |
| Date Deposited: | 31 Oct 2024 11:30 |
| Last Modified: | 01 Nov 2024 12:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97364 |
| DOI: | 10.3389/fmicb.2023.1242275 |
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