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Lu, Fu-Hao, McKenzie, Neil, Gardiner, Laura-Jayne, Luo, Ming-Cheng, Hall, Anthony 
ORCID: https://orcid.org/0000-0002-1806-020X and Bevan, Michael W.
(2020)
Reduced chromatin accessibility underlies gene expression differences in homologous chromosome arms of diploid Aegilops tauschii and hexaploid wheat.
GigaScience, 9 (6).
ISSN 2047-217X
Abstract
Background: Polyploidy is centrally important in the evolution and domestication of plants because it leads to major genomic changes, such as altered patterns of gene expression, which are thought to underlie the emergence of new traits. Despite the common occurrence of these globally altered patterns of gene expression in polyploids, the mechanisms involved are not well understood. Results: Using a precisely defined framework of highly conserved syntenic genes on hexaploid wheat chromosome 3DL and its progenitor 3 L chromosome arm of diploid Aegilops tauschii, we show that 70% of these gene pairs exhibited proportionately reduced gene expression, in which expression in the hexaploid context of the 3DL genes was ~40% of the levels observed in diploid Ae tauschii. Several genes showed elevated expression during the later stages of grain development in wheat compared with Ae tauschii. Gene sequence and methylation differences probably accounted for only a few cases of differences in gene expression. In contrast, chromosome-wide patterns of reduced chromatin accessibility of genes in the hexaploid chromosome arm compared with its diploid progenitor were correlated with both reduced gene expression and the imposition of new patterns of gene expression. Conclusions: Our pilot-scale analyses show that chromatin compaction may orchestrate reduced gene expression levels in the hexaploid chromosome arm of wheat compared to its diploid progenitor chromosome arm.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: This work was supported by BBSRC ERA-CAPS grants BB/N005104/1, BB/N005155/1 “INTREPID” to M.W.B. and A.H. M.W.B. was also supported by BBSRC Institute Strategic Programme Grants GRO (BB/J004588/1) and GEN (BB/P013511/1) to M.W.B. Publisher Copyright: © 2020 Oxford University Press. All rights reserved. |
| Uncontrolled Keywords: | atac,chr3dl,gene expression in polyploids,synteny,triticum aestivum,wheat,medicine(all) ,/dk/atira/pure/subjectarea/asjc/2700 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 31 Oct 2024 09:30 |
| Last Modified: | 31 Oct 2024 09:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97355 |
| DOI: | 10.1093/GIGASCIENCE/GIAA070 |
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