Tools
ToolsGeorge, Jeoffrey, Stegmann, Martin, Monaghan, Jacqueline, Bailey-Serres, Julia and Zipfel, Cyril (2023) Arabidopsis translation initiation factor binding protein CBE1 negatively regulates accumulation of the NADPH oxidase respiratory burst oxidase homolog D. Journal of Biological Chemistry, 299 (8). ISSN 0021-9258
Preview |
PDF (1-s2.0-S002192582302046X-main)
- Published Version
Available under License Creative Commons Attribution. Download (2MB) | Preview |
Abstract
Cell surface pattern recognition receptors sense invading pathogens by binding microbial or endogenous elicitors to activate plant immunity. These responses are under tight control to avoid excessive or untimely activation of cellular responses, which may otherwise be detrimental to host cells. How this fine-tuning is accomplished is an area of active study. We previously described a suppressor screen that identified Arabidopsis thaliana mutants with regained immune signaling in the immunodeficient genetic background bak1-5, which we named modifier of bak1-5 (mob) mutants. Here, we report that bak1-5 mob7 mutant restores elicitor-induced signaling. Using a combination of map-based cloning and whole-genome resequencing, we identified MOB7 as conserved binding of eIF4E1 (CBE1), a plant-specific protein that interacts with the highly conserved eukaryotic translation initiation factor eIF4E1. Our data demonstrate that CBE1 regulates the accumulation of respiratory burst oxidase homolog D, the NADPH oxidase responsible for elicitor-induced apoplastic reactive oxygen species production. Furthermore, several mRNA decapping and translation initiation factors colocalize with CBE1 and similarly regulate immune signaling. This study thus identifies a novel regulator of immune signaling and provides new insights into reactive oxygen species regulation, potentially through translational control, during plant stress responses.
| Item Type: | Article |
|---|---|
| Additional Information: | Data availability statement: All data are contained within the manuscript. Funding Information: This work was funded by The Gatsby Charitable Foundation (to C. Z.), The Biotechnology and Biological Research Council (BB/P012574/1), the University of Zürich (to C. Z.), and the Swiss National Science Foundation grant no. 31003A_182625 (to C. Z.). M. S. was supported by the Deutsche Forschungsgemeinschaft (Fellowship STE 2448/1) and J. M. by the European Molecular Biology Organization (Fellowships ALTF 459-2011). |
| Uncontrolled Keywords: | innate immunity,nadph oxidase,ros,signaling,translation,biochemistry,molecular biology,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1303 |
| Faculty \ School: | Faculty of Science > The Sainsbury Laboratory |
| UEA Research Groups: | Faculty of Science > Research Groups > Plant Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 31 Oct 2024 09:30 |
| Last Modified: | 07 Nov 2024 00:55 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97353 |
| DOI: | 10.1016/j.jbc.2023.105018 |
Downloads
Downloads per month over past year
Actions (login required)
 |
View Item |