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Zavan, Lauren, Fang, Haoyun, Johnston, Ella L., Whitchurch, Cynthia 
ORCID: https://orcid.org/0000-0003-2296-3791, Greening, David W., Hill, Andrew F. and Kaparakis-Liaskos, Maria
(2023)
The mechanism of Pseudomonas aeruginosa outer membrane vesicle biogenesis determines their protein composition.
Proteomics, 23 (10).
ISSN 1615-9853
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Abstract
Gram-negative bacteria produce outer membrane vesicles (OMVs) and contain bacterial cargo including nucleic acids and proteins. The proteome of OMVs can be altered by various factors including bacterial growth stage, growth conditions, and environmental factors. However, it is currently unknown if the mechanism of OMV biogenesis can determine their proteome. In this study, we examined whether the mechanisms of OMV biogenesis influenced the production and protein composition of Pseudomonas aeruginosa OMVs. OMVs were isolated from three P. aeruginosa strains that produced OMVs either by budding alone, by explosive cell lysis, or by both budding and explosive cell lysis. We identified that the mechanism of OMV biogenesis dictated OMV quantity. Furthermore, a global proteomic analysis comparing the proteome of OMVs to their parent bacteria showed significant differences in the identification of proteins in bacteria and OMVs. Finally, we determined that the mechanism of OMV biogenesis influenced the protein composition of OMVs, as OMVs released by distinct mechanisms of biogenesis differed significantly from one another in their proteome and functional enrichment analysis. Overall, our findings reveal that the mechanism of OMV biogenesis is a main factor that determines the OMV proteome which may affect their subsequent biological functions.
| Item Type: | Article |
|---|---|
| Additional Information: | Data Availability Statement: The MS-based proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository and are available via ProteomeXchange with identifier PXD032996. Funding Information: This work was supported by the Australian Research Council (project: Maria Kaparakis-Liaskos, Andrew F. Hill) under grant DP190101655. Maria Kaparakis-Liaskos was supported by a veski Inspiring Women Fellowship. This work was supported by the LIMS Bioimaging Facility (La Trobe University), and the Defence Science Institute (Lauren Zavan, Maria Kaparakis-Liaskos), an initiative of the State Government of Victoria. Open access publishing facilitated by La Trobe University, as part of the Wiley - La Trobe University agreement via the Council of Australian University Librarians. |
| Uncontrolled Keywords: | bacterial membrane vesicles,mechanism of biogenesis,outer membrane vesicles,proteome,pseudomonas aeruginosa,biochemistry,molecular biology ,/dk/atira/pure/subjectarea/asjc/1300/1303 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 29 Oct 2024 15:30 |
| Last Modified: | 31 Oct 2024 15:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97345 |
| DOI: | 10.1002/pmic.202200464 |
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