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Becker, Jemima, Sun, Bin, Alammari, Farah, Haerty, Wilfried 
ORCID: https://orcid.org/0000-0003-0111-191X, Vance, Keith W. and Szele, Francis George
(2023)
What has single-cell transcriptomics taught us about long non-coding RNAs in the ventricular-subventricular zone?
Stem Cell Reports, 18 (1).
pp. 354-376.
ISSN 2213-6711
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Abstract
Long non-coding RNA (lncRNA) function is mediated by the process of transcription or through transcript-dependent associations with proteins or nucleic acids to control gene regulatory networks. Many lncRNAs are transcribed in the ventricular-subventricular zone (V-SVZ), a postnatal neural stem cell niche. lncRNAs in the V-SVZ are implicated in neurodevelopmental disorders, cancer, and brain disease, but their functions are poorly understood. V-SVZ neurogenesis capacity declines with age due to stem cell depletion and resistance to neural stem cell activation. Here we analyzed V-SVZ transcriptomics by pooling current single-cell RNA-seq data. They showed consistent lncRNA expression during stem cell activation, lineage progression, and aging. In conjunction with epigenetic and genetic data, we predicted V-SVZ lncRNAs that regulate stem cell activation and differentiation. Some of the lncRNAs validate known epigenetic mechanisms, but most remain uninvestigated. Our analysis points to several lncRNAs that likely participate in key aspects of V-SVZ stem cell activation and neurogenesis in health and disease.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: W.H. was funded by the Biotechnology and Biological Sciences Research Council (BBSRC) Core Strategic Programme Grant BB/CSP1720/1 and its constituent work packages (BBS/E/T/000PR9818 and BBS/E/T/000PR9819). |
| Uncontrolled Keywords: | adult neurogenesis,epigenetics,lncrna,neural stem cells,scrna-seq,v-svz,biochemistry,genetics,developmental biology,cell biology,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/1300/1303 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 29 Oct 2024 15:30 |
| Last Modified: | 31 Oct 2024 15:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97341 |
| DOI: | 10.1016/j.stemcr.2022.11.011 |
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