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Sidorczuk, Katarzyna, Aieksandrowicz, Adrianna, Burdukiewicz, Michał, Kingsley, Robert A. 
ORCID: https://orcid.org/0000-0002-0194-6485 and Kolenda, Rafał
(2023)
Genomic characterization of enterohaemolysin-encoding haemolytic Escherichia coli of animal and human origin.
Microbial Genomics, 9 (4).
ISSN 2057-5858
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Abstract
Enterohaemolysin (Ehx) and alpha-haemolysin are virulence-associated factors (VAFs) causing the haemolytic phenotype in Escherichia coli. It has been shown that chromosomally and plasmid-encoded alpha-haemolysin are characteristic of specific pathotypes, virulence-associated factors and hosts. However, the prevalence of alpha- and enterohaemolysin does not overlap in the majority of pathotypes. Therefore, this study focuses on the characterization of the haemolytic E. coli population associated with multiple pathotypes in human and animal infectious diseases. Using a genomics approach, we investigated characteristic features of the enterohaemolysin-encoding strains to identity factors differentiating enterohaemolysin-positive trom alpha-haemolysin-positive E. coli populations. To shed light on the functionality of Ehx subtypes, we analysed Ehx-coding genes and interred EhxA phylogeny. The two haemolysins are associated with a different repertoire of adhesins, iron acquisition or toxin systems. Alpha-haemolysin is predominantly found in uropathogenic E. coli (UPEC) and predicted to be chromosomally encoded, or nonpathogenic and undetermined E. coli pathotypes and typically predicted to be plasmid-encoded. Enterohae-molysin is mainly associated with Shiga toxin-producing E. coli (STEC) and enterohaemorrhagic E. coli (EHEC) and predicted to be plasmid-encoded. Both types of haemolysin are found in atypical enteropathogenic E. coli (aEPEC). Moreover, we identified a new EhxA subtype present exclusively in genomes with VAFs characteristic of nonpathogenic E. coli. This study reveals a complex relationship between haemolytic E. coli of diverse pathotypes, providing a framework tor understanding the potential role of haemolysin in pathogenesis.
| Item Type: | Article |
|---|---|
| Additional Information: | Data Summary: The authors confirm all supporting data, code and protocols have been provided within the article or through supplementary data files. Supplementary data containing all supplementary figures and tables (Supplementary Material). Dataset S1: list of genomes used in this study (Supplementary Dataset S1). Supplementary dataset 2: results of mapping of the contigs identified as plasmids by Platon to 306 reference plasmids from PLSDB containing enterohaemolysin (Supplementary Dataset S2). Funding Information: R.A.K. was supported by the UKRI Institute Strategic Programme Microbes in the Food Chain BB/R012504/1 and its constituent project(s) BBS/E/ F/000PR10348 and BBS/E/F/000PR10349. M.B. was supported by the Maria Zambrano grant funded by the European Union-NextGenerationEU. K.S. was supported by the National Science Centre grant 2018/31/N/NZ2/01338. |
| Uncontrolled Keywords: | adhesins,alpha-haemolysin,ehxa,enterohaemolysin,escherichia coli,haemolytic,iron acquisition,toxin,epidemiology,microbiology,molecular biology,genetics,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2700/2713 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 29 Oct 2024 14:30 |
| Last Modified: | 27 Nov 2024 10:44 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97338 |
| DOI: | 10.1099/MGEN.0.000999 |
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