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ToolsStentz, Régis, Cheema, Jitender, Philo, Mark and Carding, Simon R. (2023) A possible aquatic origin of the Thiaminase TenA of the human gut symbiont Bacteroides thetaiotaomicron. Journal of Molecular Evolution, 91 (4). pp. 482-491. ISSN 0022-2844
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Abstract
TenA thiamin-degrading enzymes are commonly found in prokaryotes, plants, fungi and algae and are involved in the thiamin salvage pathway. The gut symbiont Bacteroides thetaiotaomicron (Bt) produces a TenA protein (BtTenA) which is packaged into its extracellular vesicles. An alignment of BtTenA protein sequence with proteins from different databases using the basic local alignment search tool (BLAST) and the generation of a phylogenetic tree revealed that BtTenA is related to TenA-like proteins not only found in a small number of intestinal bacterial species but also in some aquatic bacteria, aquatic invertebrates, and freshwater fish. This is, to our knowledge, the first report describing the presence of TenA-encoding genes in the genome of members of the animal kingdom. By searching metagenomic databases of diverse host-associated microbial communities, we found that BtTenA homologues were mostly represented in biofilms present on the surface of macroalgae found in Australian coral reefs. We also confirmed the ability of a recombinant BtTenA to degrade thiamin. Our study shows that BttenA-like genes which encode a novel sub-class of TenA proteins are sparingly distributed across two kingdoms of life, a feature of accessory genes known for their ability to spread between species through horizontal gene transfer.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: This research was funded by the Biotechnology and Biological Sciences Research Council (BBSRC) Institute Strategic Programme Grant Gut Microbes and Health BB/R012490/1 and its constituent project(s) BBS/E/F/000PR10353 (Theme 1, Determinants of microbe-host responses in the gut across life), and the BBSRC Core Capability Grant BB/CCG1860/1. |
| Uncontrolled Keywords: | bacterial extracellular vesicles,horizontal gene transfer,monophyletic group,thiamin,ecology, evolution, behavior and systematics,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/1100/1105 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 29 Oct 2024 14:30 |
| Last Modified: | 04 Nov 2024 12:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97335 |
| DOI: | 10.1007/s00239-023-10101-8 |
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