Cell-specific RNA profiling reveals host genes expressed in Arabidopsis cells haustoriated by downy mildew

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Asai, Shuta, Cevik, Volkan, Jones, Jonathan D. G. and Shirasu, Ken (2023) Cell-specific RNA profiling reveals host genes expressed in Arabidopsis cells haustoriated by downy mildew. Plant Physiology, 193 (1). pp. 259-270. ISSN 0032-0889

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Abstract

The downy mildew oomycete Hyaloperonospora arabidopsidis, an obligate filamentous pathogen, infects Arabidopsis (Arabidopsis thaliana) by forming structures called haustoria inside host cells. Previous transcriptome analyses have revealed that host genes are specifically induced during infection; however, RNA profiling from whole-infected tissues may fail to capture key transcriptional events occurring exclusively in haustoriated host cells, where the pathogen injects virulence effectors to modulate host immunity. To determine interactions between Arabidopsis and H. arabidopsidis at the cellular level, we devised a translating ribosome affinity purification system using 2 high-affinity binding proteins, colicin E9 and Im9 (immunity protein of colicin E9), applicable to pathogen-responsive promoters, thus enabling haustoriated cell-specific RNA profiling. Among the host genes specifically expressed in H. arabidopsidis–haustoriated cells, we found genes that promote either susceptibility or resistance to the pathogen, providing insights into the Arabidopsis–downy mildew interaction. We propose that our protocol for profiling cell-specific transcripts will apply to several stimulus-specific contexts and other plant–pathogen interactions.

Item Type:	Article
Additional Information:	Funding Information: This work was supported by JSPS KAKENHI Grant Numbers 17K07679 (S.A.), 20H02995 (S.A.), JP20H05909 (K.S.), and JP22H00364 (K.S.) and by funding to TSL from the Gatsby Foundation (J.D.G.J).
Uncontrolled Keywords:	physiology,genetics,plant science ,/dk/atira/pure/subjectarea/asjc/1300/1314
Faculty \ School:	Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Plant Sciences
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	29 Oct 2024 14:30
Last Modified:	04 Nov 2024 12:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/97333
DOI:	10.1093/plphys/kiad326

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