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Greenwood, Mark, Hall, Anthony J.W. 
ORCID: https://orcid.org/0000-0002-1806-020X and Locke, James C.W.
(2022)
High Spatial Resolution Luciferase Imaging of the Arabidopsis thaliana Circadian Clock.
In:
Plant Circadian Networks.
Methods in Molecular Biology
(2).
Humana Press Inc, pp. 47-55.
ISBN 978-1-0716-1911-7
Abstract
The A. thaliana circadian clock is an example of a gene network that generates rich temporal and spatial dynamics. Bioluminescent imaging has proven a powerful method to help dissect the genetic mechanisms that generate oscillations of gene expression over the course of the day. However, its use for the study of spatial regulation is often limited by resolution. Here, we describe a modified luciferase imaging method for the study of the Arabidopsis circadian clock across the plant at sub-tissue-level resolution.
| Item Type: | Book Section |
|---|---|
| Additional Information: | Funding Information: M.G. and J.L. are funded by the Gatsby Charitable Foundation (grant number GAT3395/GLC). A.H. is funded by the BBSRC (grant number BB/P016774/1). Publisher Copyright: © 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. |
| Uncontrolled Keywords: | circadian clock,coordination,high resolution,imaging,luciferase,spatial,tissue specific,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/1300/1312 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 29 Oct 2024 11:30 |
| Last Modified: | 29 Oct 2024 11:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97307 |
| DOI: | 10.1007/978-1-0716-1912-4_4 |
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