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ToolsRhodes, Jack and Zipfel, Cyril (2024) Identification of Bioactive Phytocytokines Using Transcriptomic Data and Plant Bioassays. In: Plant Peptide Hormones and Growth Factors. Methods in Molecular Biology (1). Humana Press Inc, pp. 23-35. ISBN 978-1-0716-3510-0
Full text not available from this repository. (Request a copy)Abstract
Plant genomes contain thousands of short open reading frames that encode putative peptides. Some of these peptides play important signaling roles in response to environmental stress. Here we describe a pipeline used to identify the CTNIP/SMALL PHYTOCYTOKINES REGULATING DEFENSE AND WATER LOSS (SCREW) family of phytocytokines, based upon their transcriptional upregulation during biotic stress. Moreover, we describe approaches to assay their activity in planta by measuring increases in cytoplasmic calcium concentration, reactive oxygen species production, and mitogen-activated protein kinase phosphorylation.
| Item Type: | Book Section |
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| Additional Information: | Funding Information: We thank all members of the Zipfel group for fruitful discussionswell as the plant signaling peptide community. The authoacknowledge generous funding to study plant immune signalinby the Gatsby Charitable Foundation (CZ), the Biotechnology anBiological Research Council (BB/P012574/1) (CZ), the European Research Council under the European Union’s Horizo2020 research and innovation program no. 773153, proje“IMMUNO-PEPTALK” (CZ), the University of Zurich (CZ)and the Swiss National Science Foundation granno. 31003A_182625 (CZ). Publisher Copyright: © 2024, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. |
| Uncontrolled Keywords: | calcium influx,immunity,mitogen-activated protein kinase,peptide signaling,phytocytokine,reactive oxygen species,transcriptomics,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/1300/1312 |
| Faculty \ School: | Faculty of Science > The Sainsbury Laboratory |
| UEA Research Groups: | Faculty of Science > Research Groups > Plant Sciences |
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| Depositing User: | LivePure Connector |
| Date Deposited: | 29 Oct 2024 11:30 |
| Last Modified: | 29 Oct 2024 11:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97306 |
| DOI: | 10.1007/978-1-0716-3511-7_2 |
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