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Nichols, Claire M., Povstyan, Oleksandr V., Albert, Anthony P. 
ORCID: https://orcid.org/0000-0002-3596-9634, Gordienko, Dmitry V., Khan, Omar, Vasilikostas, Georgios, Khong, Teck K., Wan, Andrew, Reddy, Marcus and Harhun, Maksym I.
(2014)
Vascular smooth muscle cells from small human omental arteries express P2X1 and P2X4 receptor subunits.
Purinergic Signalling, 10 (4).
pp. 565-572.
ISSN 1573-9538
Abstract
Stimulation of P2X receptors by ATP in vascular smooth muscle cells (VSMCs) is proposed to mediate vascular tone. However, understanding of P2X receptor-mediated actions in human blood vessels is limited, and therefore, the current work investigates the role of P2X receptors in freshly isolated small human gastro-omental arteries (HGOAs). Expression of P2X1 and P2X4 receptor subunit messenger RNA (mRNA) and protein was identified in individual HGOA VSMCs using RT-PCR and immunofluorescent analysis and using Western blot in multi-cellular preparations. ATP of 10 μmol/l and αβ-meATP of 10 μmol/l, a selective P2X receptor agonist, evoked robust increases in [Ca2+]i in fluo-3-loaded HGOA VSMCs. Pre-incubation with 1 μmol/l NF279, a selective P2X receptor antagonist, reduced the amplitude of αβ-meATP-induced increase in [Ca2+]i by about 70 %. ATP of 10 μmol/l and αβ-meATP of 10 μmol/l produced similar contractile responses in segments of HGOA, and these contractions were greatly reduced by 2 μmol/l NF449, a selective P2X receptor inhibitor. These data suggest that VSMCs from HGOA express P2X1 and P2X4 receptor subunits with homomeric P2X1 receptors likely serving as the predominant target for extracellular ATP.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: Acknowledgments This work was supported by the British Heart Foundation Intermediate Basic Science Research Fellowship to M.I.H. (FS/06/077) and BHF grant (PG/08/062/) to D.V.G. and M.I.H. Publisher Copyright: © 2014, Springer Science+Business Media Dordrecht. |
| Uncontrolled Keywords: | atp,gastro-omental arteries,human,nf279,p2x1 receptor,molecular biology,cellular and molecular neuroscience,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1312 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 29 Oct 2024 09:30 |
| Last Modified: | 03 Nov 2024 07:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97251 |
| DOI: | 10.1007/s11302-014-9415-6 |
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