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Liu, Hang, Lu, Xunli, Li, Mengfei, Lun, Zhiqin, Yan, Xia, Yin, Changfa, Yuan, Guixin, Wang, Xingbin, Liu, Ning, Liu, Di, Wu, Mian, Luo, Ziluolong, Zhang, Yan, Bhadauria, Vijai, Yang, Jun, Talbot, Nicholas J. 
ORCID: https://orcid.org/0000-0001-6434-7757 and Peng, You Liang
(2023)
Plant immunity suppression by an exo-β-1,3-glucanase and an elongation factor 1α of the rice blast fungus.
Nature Communications, 14.
ISSN 2041-1723
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Abstract
Fungal cell walls undergo continual remodeling that generates β-1,3-glucan fragments as products of endo-glycosyl hydrolases (GHs), which can be recognized as pathogen-associated molecular patterns (PAMPs) and trigger plant immune responses. How fungal pathogens suppress those responses is often poorly understood. Here, we study mechanisms underlying the suppression of β-1,3-glucan-triggered plant immunity by the blast fungus Magnaporthe oryzae. We show that an exo-β-1,3-glucanase of the GH17 family, named Ebg1, is important for fungal cell wall integrity and virulence of M. oryzae. Ebg1 can hydrolyze β-1,3-glucan and laminarin into glucose, thus suppressing β-1,3-glucan-triggered plant immunity. However, in addition, Ebg1 seems to act as a PAMP, independent of its hydrolase activity. This Ebg1-induced immunity appears to be dampened by the secretion of an elongation factor 1 alpha protein (EF1α), which interacts and co-localizes with Ebg1 in the apoplast. Future work is needed to understand the mechanisms behind Ebg1-induced immunity and its suppression by EF1α.
| Item Type: | Article |
|---|---|
| Additional Information: | Data availability statement: The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the iProX partner repository with the dataset identifier PXD024706 and PXD044578. Source data are provided with this paper. Funding Information: This work was supported by the National Key Research and Development Program from Ministry of Science and Technology, China [grant 2016YFD0300703] to Y.-L.P., the National Natural Science Foundation of China [Grant No. 32172370] to X.L., the China Agricultural Research System from Ministry of Agriculture and Rural Affairs [CARS-01-43], the CSIRT Program [IRT1042] and the 111 Project [B13006] from Ministry of Education, China, and the Pinduoduo-China Agricultural University Research Fund to Y.-L.P. N.J.T. is funded by the Gatsby Charitable Foundation, the Biotechnology and Biological Sciences Research Council (BBS/E/J/000PR9797) and is a partner in 111 Project [B13006]. The funders had no role in study design, data collection and analysis, the decision to publish, or preparation of the manuscript. |
| Uncontrolled Keywords: | chemistry(all),biochemistry, genetics and molecular biology(all),physics and astronomy(all) ,/dk/atira/pure/subjectarea/asjc/1600 |
| Faculty \ School: | Faculty of Science > The Sainsbury Laboratory |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 25 Oct 2024 14:30 |
| Last Modified: | 30 Oct 2024 00:52 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97221 |
| DOI: | 10.1038/s41467-023-41175-z |
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