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Lind, Martin I., Mautz, Brian S., Carlsson, Hanne, Hinas, Andrea, Gudmunds, Erik and Maklakov, Alexei A. 
ORCID: https://orcid.org/0000-0002-5809-1203
(2024)
Sex-specific growth and lifespan effects of germline removal in the dioecious nematode Caenorhabditis remanei.
Aging Cell, 23 (11).
ISSN 1474-9718
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Abstract
Germline regulates the expression of life-history traits and mediates the trade-off between reproduction and somatic maintenance. However, germline maintenance in itself can be costly, and the costs can vary between the sexes depending on the number of gametes produced across the lifetime. We tested this directly by germline ablation using glp-1 RNA interference (RNAi) in a dioecious nematode Caenorhabditis remanei. Germline removal strongly increased heat-shock resistance in both sexes, thus confirming the role of the germline in regulating somatic maintenance. However, germline removal resulted in increased lifespan only in males. High costs of mating strongly reduced lifespan in both sexes and obliterated the survival benefit of germline-less males even though neither sex produced any offspring. Furthermore, germline removal reduced male growth before maturation but not in adulthood, while female growth rate was reduced both before and especially after maturation. Thus, germline removal improves male lifespan without major growth costs, while germline-less females grow slower and do not live longer than reproductively functional counterparts in the absence of environmental stress. Overall, these results suggest that germline maintenance is costlier for males than for females in C. remanei.
| Item Type: | Article |
|---|---|
| Additional Information: | Data Availability Statement: The data that support the findings of this study are openly available in Figshare at http://doi.org/10.6084/m9.figshare.25053644. Funding Information: This work was supported by the European Research Council [grants St\u2010G AGINGSEXDIFF 260885 and Co\u2010G GermlineAgeingSoma 724,909 to AAM] and the Swedish Research Council [grant no. 2020\u201304388 to MIL]. |
| Uncontrolled Keywords: | germline,glp-1,heat-shock,lifespan,sex-specific,ageing,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1302 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging Faculty of Science > Research Centres > Centre for Ecology, Evolution and Conservation Faculty of Science > Research Groups > Organisms and the Environment |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 25 Oct 2024 10:30 |
| Last Modified: | 18 Nov 2024 12:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97208 |
| DOI: | 10.1111/acel.14290 |
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