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Sayer, Andrew P., Llavero-Pasquina, Marcel, Geisler, Katrin, Holzer, Andre, Bunbury, Freddy, Mendoza-Ochoa, Gonzalo I., Lawrence, Andrew D., Warren, Martin J. 
ORCID: https://orcid.org/0000-0002-6028-6456, Mehrshahi, Payam and Smith, Alison G.
(2024)
Conserved cobalamin acquisition protein 1 is essential for vitamin B12 uptake in both Chlamydomonas and Phaeodactylum.
Plant Physiology, 194 (2).
pp. 698-714.
ISSN 0032-0889
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Abstract
Microalgae play an essential role in global net primary productivity and global biogeochemical cycling. Despite their phototrophic lifestyle, over half of algal species depend for growth on acquiring an external supply of the corrinoid vitamin B12 (cobalamin), a micronutrient produced only by a subset of prokaryotic organisms. Previous studies have identified protein components involved in vitamin B12 uptake in bacterial species and humans. However, little is known about its uptake in algae. Here, we demonstrate the essential role of a protein, cobalamin acquisition protein 1 (CBA1), in B12 uptake in Phaeodactylum tricornutum using CRISPR-Cas9 to generate targeted knockouts and in Chlamydomonas reinhardtii by insertional mutagenesis. In both cases, CBA1 knockout lines could not take up exogenous vitamin B12. Complementation of the C. reinhardtii mutants with the wild-type CBA1 gene restored B12 uptake, and regulation of CBA1 expression via a riboswitch element enabled control of the phenotype. When visualized by confocal microscopy, a YFP-fusion with C. reinhardtii CBA1 showed association with membranes. Bioinformatics analysis found that CBA1-like sequences are present in all major eukaryotic phyla. In algal taxa, the majority that encoded CBA1 also had genes for B12-dependent enzymes, suggesting CBA1 plays a conserved role. Our results thus provide insight into the molecular basis of algal B12 acquisition, a process that likely underpins many interactions in aquatic microbial communities.
| Item Type: | Article |
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| Additional Information: | Funding Information: This work was supported by the Biotechnology and Biological Sciences Research Council of the UK (BBSRC) Doctoral Training Partnership, grant no. BB/M011194/1 to A.P.S., M.L.P., and A.G.S.; grant no. BB/M018180/1 to P.M. and A.G.S.; grant no. BB/L002957/1 and BB/R021694/1 to K.G. and A.G.S.; grant no. BB/L014130/1 to G.I.M, K.G., P.M., and A.G.S.; grant no. BB/S002197/1 to M.J.W.; University of Cambridge Broodbank Fellowship to G.I.M; Royal Society grant no. INF\R2\180062 to M.J.W.; and Bill and Melinda Gates Foundation grant OPP1144 and Gates Cambridge Trust (Graduate Student Fellowship) to A.H. |
| Uncontrolled Keywords: | physiology,genetics,plant science ,/dk/atira/pure/subjectarea/asjc/1300/1314 |
| Faculty \ School: | Faculty of Science Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 24 Oct 2024 14:30 |
| Last Modified: | 01 Nov 2024 13:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97180 |
| DOI: | 10.1093/plphys/kiad564 |
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