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Goto, Yukihisa, Maki, Noriko, Sklenar, Jan, Derbyshire, Paul, Menke, Frank L. H. 
ORCID: https://orcid.org/0000-0003-2490-4824, Zipfel, Cyril, Kadota, Yasuhiro and Shirasu, Ken
(2024)
The phagocytosis oxidase/Bem1p domain-containing protein PB1CP negatively regulates the NADPH oxidase RBOHD in plant immunity.
New Phytologist, 241 (4).
pp. 1763-1779.
ISSN 0028-646X
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Abstract
Perception of pathogen-associated molecular patterns (PAMPs) by surface-localized pattern recognition receptors activates RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD) through direct phosphorylation by BOTRYTIS-INDUCED KINASE 1 (BIK1) and induces the production of reactive oxygen species (ROS). RBOHD activity must be tightly controlled to avoid the detrimental effects of ROS, but little is known about RBOHD downregulation. To understand the regulation of RBOHD, we used co-immunoprecipitation of RBOHD with mass spectrometry analysis and identified PHAGOCYTOSIS OXIDASE/BEM1P (PB1) DOMAIN-CONTAINING PROTEIN (PB1CP). PB1CP negatively regulates RBOHD and the resistance against the fungal pathogen Colletotrichum higginsianum. PB1CP competes with BIK1 for binding to RBOHD in vitro. Furthermore, PAMP treatment enhances the PB1CP-RBOHD interaction, thereby leading to the dissociation of phosphorylated BIK1 from RBOHD in vivo. PB1CP localizes at the cell periphery and PAMP treatment induces relocalization of PB1CP and RBOHD to the same small endomembrane compartments. Additionally, overexpression of PB1CP in Arabidopsis leads to a reduction in the abundance of RBOHD protein, suggesting the possible involvement of PB1CP in RBOHD endocytosis. We found PB1CP, a novel negative regulator of RBOHD, and revealed its possible regulatory mechanisms involving the removal of phosphorylated BIK1 from RBOHD and the promotion of RBOHD endocytosis.
| Item Type: | Article |
|---|---|
| Additional Information: | Data availability statement: The data that support the findings of this study are available in the Supporting Information of this article. Funding Information: The research was financially supported by JSPS KAKENHI Grant no. 16 J00771 (to Y.G.), 16H06186, 16KT0037, 20H02994, 21 K19128 (to Y.K), 15H05959, 17H06172, 22H00364 (to K.S), as well as the Gatsby Charitable Foundation (to F.L.H.M and C.Z.) and the European Research Council (project ‘PHOSPHinnATE’, grant agreement no. 309858 to C.Z.). |
| Uncontrolled Keywords: | arabidopsis,nadph oxidase respiratory burst oxidase homolog d,pathogen-associated molecular patterns,pattern recognition receptor-triggered immunity,reactive oxygen species,physiology,plant science ,/dk/atira/pure/subjectarea/asjc/1300/1314 |
| Faculty \ School: | Faculty of Science > The Sainsbury Laboratory Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Plant Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 24 Oct 2024 09:30 |
| Last Modified: | 17 Dec 2024 01:41 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97176 |
| DOI: | 10.1111/nph.19302 |
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