Cutin-derived oligomers induce hallmark plant immune responses

Moreira, Carlos J. S., Escórcio, Rita, Bento, Artur, Bjornson, Marta, Herold, Laura, Tomé, Ana S., Martins, Celso, Fanuel, Mathieu, Martins, Isabel, Bakan, Bénédicte, Zipfel, Cyril and Silva Pereira, Cristina (2024) Cutin-derived oligomers induce hallmark plant immune responses. Journal of Experimental Botany, 75 (16). pp. 5146-5161. ISSN 0022-0957

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Abstract

The cuticle constitutes the outermost defensive barrier of most land plants. It comprises a polymeric matrix - cutin, surrounded by soluble waxes. Moreover, the cuticle constitutes the first line of defense against pathogen invasion, while also protecting the plant from many abiotic stresses. Aliphatic monomers in cutin have been suggested to act as immune elicitors in plants. This study analyses the potential of cutin oligomers to activate rapid signaling outputs reminiscent of pattern-triggered immunity in the model plant Arabidopsis. Cutin oligomeric mixtures led to Ca2+ influx and mitogen-activated protein kinase activation. Comparable responses were measured for cutin, which was also able to induce a reactive oxygen species burst. Furthermore, cutin oligomer treatment resulted in a unique transcriptional reprogramming profile, having many archetypal features of pattern-triggered immunity. Targeted spectroscopic and spectrometric analyses of the cutin oligomers suggest that the elicitor compounds consist mostly of two up to three 10,16-dihydroxyhexadecanoic acid monomers linked together through ester bonds. This study demonstrates that cutin breakdown products can act as inducers of early plant immune responses. Further investigation is needed to understand how cutin breakdowns are perceived and to explore their potential use in agriculture.

Item Type:	Article
Additional Information:	Data availability statement: All relevant data are available in the manuscript. Supplementary figures and tables are provided to support the results presented. The transcriptomic data have been deposited in the BioStudies EMBL-EBI platform with the accession number E-MTAB-12956, and are also accessible through the SRA NCBI public database with the same accession number. Funding Information: European Research Council through grant ERC 2014-CoG-647928 and from Funda\u00E7\u00E3o para a Ci\u00EAncia e Tecnologia (FCT) by Project MOSTMICRO ITQB (UIDB/04612/2020 and UIDP/04612/2020), LS4FUTURE Associated Laboratory (LA/P/0087/2020), from the PESSOA program (Prpc no. 441.00) to CSP, and from the University of Zurich to CZ. The NMR data were acquired at CERMAX, ITQB-NOVA, Oeiras, Portugal with equipment funded by FCT. CJSM is grateful to Aralab (Portugal) for the PhD contract 06/PlantsLife/2017 and to EMBO for the short-term fellowship (#8003). RE and IM are grateful for FCT funding for the PhD scholarship BD/06435/2021 and for the working contract financed by national funds under norma transit\u00F3ria D.L. no. 57/2016, respectively.
Uncontrolled Keywords:	arabidopsis thaliana,calcium influx,cuticle,cutin oligomers,mapk activation,nicotiana benthamiana,pattern-triggered immunity (pti),rna-seq,solanum lycopersicum,physiology,plant science ,/dk/atira/pure/subjectarea/asjc/1300/1314
Faculty \ School:	Faculty of Science > The Sainsbury Laboratory
UEA Research Groups:	Faculty of Science > Research Groups > Plant Sciences
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	24 Oct 2024 08:30
Last Modified:	29 Oct 2024 00:53
URI:	https://ueaeprints.uea.ac.uk/id/eprint/97172
DOI:	10.1093/jxb/erae254

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