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Zdrzałek, Rafał, Xi, Yuxuan, Langner, Thorsten, Bentham, Adam R., Petit-Houdenot, Yohann, De la Concepcion, Juan Carlos, Harant, Adeline, Shimizu, Motoki, Were, Vincent, Talbot, Nicholas J. 
ORCID: https://orcid.org/0000-0001-6434-7757, Terauchi, Ryohei, Kamoun, Sophien ORCID: https://orcid.org/0000-0002-0290-0315 and Banfield, Mark J.
(2024)
Bioengineering a plant NLR immune receptor with a robust binding interface toward a conserved fungal pathogen effector.
Proceedings of the National Academy of Sciences of the United States of America, 121 (28).
ISSN 0027-8424
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Abstract
Bioengineering of plant immune receptors has emerged as a key strategy for generating novel disease resistance traits to counteract the expanding threat of plant pathogens to global food security. However, current approaches are limited by rapid evolution of plant pathogens in the field and may lack durability when deployed. Here, we show that the rice nucleotide-binding, leucine-rich repeat (NLR) immune receptor Pik-1 can be engineered to respond to a conserved family of effectors from the multihost blast fungus pathogen Magnaporthe oryzae. We switched the effector binding and response profile of the Pik NLR from its cognate rice blast effector AVR-Pik to the host-determining factor pathogenicity toward weeping lovegrass 2 (Pwl2) by installing a putative host target, OsHIPP43, in place of the native integrated heavy metal–associated domain (generating Pikm-1OsHIPP43). This chimeric receptor also responded to other PWL alleles from diverse blast isolates. The crystal structure of the Pwl2/OsHIPP43 complex revealed a multifaceted, robust interface that cannot be easily disrupted by mutagenesis, and may therefore provide durable, broad resistance to blast isolates carrying PWL effectors in the field. Our findings highlight how the host targets of pathogen effectors can be used to bioengineer recognition specificities that have more robust properties compared to naturally evolved disease resistance genes.
| Item Type: | Article |
|---|---|
| Additional Information: | Data, Materials, and Software Availability: All study data are included in the article and/or SI Appendix. Funding Information: United Kingdom Research and Innovation (UKRI) Biotechnology and Biological Sciences Research Council (BBSRC, UK, grants BB/ W00108X/1, BB/WW002221/1, BB/V002937/1, BB/V016342, BB/P012574, BBS/ E/J/000PR9795, BBS/E/J/000PR9796, and BB/X010996/1), UKRI-BBSRC Norwich Research Park Biosciences Doctoral Training Partnership (grant BB/M011216/1), the European Research Council (743165 \u201CBLASTOFF,\u201D 101077853 \u201CPANDEMIC\u201D), the John Innes Foundation, the Gatsby Charitable Foundation, and the Japanese Society for the Promotion of Science (JSPS, KAKENHI 20H05681, 23K20042). |
| Uncontrolled Keywords: | integrated domain,nlr,plant immunity,protein structure,recognition |
| Faculty \ School: | Faculty of Science > The Sainsbury Laboratory Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging Faculty of Science > Research Groups > Plant Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 24 Oct 2024 08:30 |
| Last Modified: | 17 Dec 2024 01:41 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97171 |
| DOI: | 10.1073/pnas.2402872121 |
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