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De la Concepcion, Juan Carlos, Langner, Thorsten, Fujisaki, Koki, Yan, Xia, Were, Vincent, Ching Lam, Anson Ho, Saado, Indira, Brabham, Helen J., Win, Joe, Yoshida, Kentaro, Talbot, Nicholas J. 
ORCID: https://orcid.org/0000-0001-6434-7757, Terauchi, Ryohei, Kamoun, Sophien ORCID: https://orcid.org/0000-0002-0290-0315 and Banfield, Mark J.
(2024)
Zinc-finger (ZiF) fold secreted effectors form a functionally diverse family across lineages of the blast fungus Magnaporthe oryzae.
PLoS Pathogens, 20 (6).
ISSN 1553-7366
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Abstract
Filamentous plant pathogens deliver effector proteins into host cells to suppress host defence responses and manipulate metabolic processes to support colonization. Understanding the evolution and molecular function of these effectors provides knowledge about pathogenesis and can suggest novel strategies to reduce damage caused by pathogens. However, effector proteins are highly variable, share weak sequence similarity and, although they can be grouped according to their structure, only a few structurally conserved effector families have been functionally characterized to date. Here, we demonstrate that Zinc-finger fold (ZiF) secreted proteins form a functionally diverse effector family in the blast fungus Magnaporthe oryzae. This family relies on the Zinc-finger motif for protein stability and is ubiquitously present in blast fungus lineages infecting 13 different host species, forming different effector tribes. Homologs of the canonical ZiF effector, AVR-Pii, from rice infecting isolates are present in multiple M. oryzae lineages. Wheat infecting strains of the fungus also possess an AVR-Pii like allele that binds host Exo70 proteins and activates the immune receptor Pii. Furthermore, ZiF tribes may vary in the proteins they bind to, indicating functional diversification and an intricate effector/host interactome. Altogether, we uncovered a new effector family with a common protein fold that has functionally diversified in lineages of M. oryzae. This work expands our understanding of the diversity of M. oryzae effectors, the molecular basis of plant pathogenesis and may ultimately facilitate the development of new sources for pathogen resistance.
| Item Type: | Article |
|---|---|
| Additional Information: | Data Availability Statement: The authors confirm that all data underlying the findings are fully available without restriction. All relevant data are within the paper and its Supporting Information files. Funding Information: This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC, UK, grants BB/WW002221/1 and BB/V002937/1 to SK; BB/V016342 to NJT; BB/ V015508/1 to MJB; BB/P012574 to MJB, SK and NJT; BBS/E/J/000PR9795 to MJB, SK and NJT; BBS/E/J/000PR9796 to MJB, SK and NJT; and BB/ X010996/1 to MJB, SK and NJT). This work was also supported by The European Research Council (743165, \u201CBLASTOFF\u201D to SK and MJB); by The John Innes Foundation to JCDlC and AL; by The Gatsby Charitable Foundation to SK and NJT; and by The Japanese Society for the Promotion of Science (JSPS, KAKENHI 20H05681 to RT).The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
| Uncontrolled Keywords: | parasitology,microbiology,immunology,molecular biology,genetics,virology ,/dk/atira/pure/subjectarea/asjc/2400/2405 |
| Faculty \ School: | Faculty of Science > The Sainsbury Laboratory Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging Faculty of Science > Research Groups > Plant Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 23 Oct 2024 11:30 |
| Last Modified: | 07 Nov 2024 00:55 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97147 |
| DOI: | 10.1371/journal.ppat.1012277 |
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