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Aleksandrowicz, Adrianna, Kjærup, Rikke Brødsgaard, Grzymajło, Krzysztof, Martinez, Fernando Garcia, Muñoz, Javier, Borowska, Dominika, Sives, Samantha, Vervelde, Lonneke, Dalgaard, Tina Sørensen, Kingsley, Robert A. 
ORCID: https://orcid.org/0000-0002-0194-6485 and Kolenda, Rafał
(2024)
FdeC expression regulates motility and adhesion of the avian pathogenic Escherichia coli strain IMT5155.
Veterinary Research, 55.
p. 70.
ISSN 1297-9716
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Abstract
Adaptation of avian pathogenic E. coli (APEC) to changing host environments including virulence factors expression is vital for disease progression. FdeC is an autotransporter adhesin that plays a role in uropathogenic Escherichia coli (UPEC) adhesion to epithelial cells. Expression of fdeC is known to be regulated by environmental conditions in UPEC and Shiga toxin-producing E. coli (STEC). The observation in a previous study that an APEC strain IMT5155 in which the fdeC gene was disrupted by a transposon insertion resulted in elevated adhesion to chicken intestinal cells prompted us to further explore the role of fdeC in infection. We found that the fdeC gene prevalence and FdeC variant prevalence differed between APEC and nonpathogenic E. coli genomes. Expression of the fdeC gene was induced at host body temperature, an infection relevant condition. Disruption of fdeC resulted in greater adhesion to CHIC-8E11 cells and increased motility at 42 °C compared to wild type (WT) and higher expression of multiple transporter proteins that increased inorganic ion export. Increased motility may be related to increased inorganic ion export since this resulted in downregulation of YbjN, a protein known to supress motility. Inactivation of fdeC in APEC strain IMT5155 resulted in a weaker immune response in chickens compared to WT in experimental infections. Our findings suggest that FdeC is upregulated in the host and contributes to interactions with the host by down-modulating motility during colonization. A thorough understanding of the regulation and function of FdeC could provide novel insights into E. coli pathogenesis.
| Item Type: | Article |
|---|---|
| Additional Information: | Availability of data and materials: All data generated or analysed during this study are included in this published article and its supplementary information files. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD045075. Funding information: This work was supported by VetBioNet, a European Union’s Horizon 2020 research and innovation program, grant agreement No 731014 (Ref: VBN_20_49) and by EPIC-XS, project number 823839, funded by the Horizon 2020 programme of the European Union. The work of AA was supported by the Polish National Science Centre Research Grant PRELUDIUM BIS [UMO-2019/35/O/NZ6/01590]. AA was supported by a Houghton Trust travel grant to visit the Roslin Institute. LV was supported by the Biotechnology and Biological Sciences Research Council Institute Strategic Program Grant funding (BBS/E/D/10002073 and BBS/E/D/20002174). The author(s) gratefully acknowledge the support of the Biotechnology and Biological Sciences Research Council (BBSRC); this research was funded by the BBSRC Institute Strategic Programme Microbes in the Food Chain BB/R012504/1 and its constituent projects BBS/E/F/000PR10348 and BBS/E/F/000PR10349 and the BBSRC Institute Strategic Programme Microbes and Food Safety BB/X011011/1 and its constituent projects, BBS/E/F/000PR13635 and BBS/E/F/000PR13636. The APC/BPC is financed by Wrocław University of Environmental and Life Sciences. |
| Uncontrolled Keywords: | escherichia coli,adhesin,adhesion,apec,autotransporter,fdec,motility,proteomics,regulation,medicine(all) ,/dk/atira/pure/subjectarea/asjc/2700 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 23 Oct 2024 11:30 |
| Last Modified: | 05 Dec 2024 01:31 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97144 |
| DOI: | 10.1186/s13567-024-01327-5 |
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