Tools
Tools
Kourelis, Jiorgos, Schuster, Mariana, Demir, Fatih, Mattinson, Oliver, Krauter, Sonja, Kahlon, Parvinderdeep S., O’Grady, Ruby, Royston, Samantha, Bravo-Cazar, Ana Lucía, Mooney, Brian C., Huesgen, Pitter F., Kamoun, Sophien 
ORCID: https://orcid.org/0000-0002-0290-0315 and van der Hoorn, Renier A. L.
(2024)
Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors.
Plant Cell, 36 (9).
pp. 3260-3276.
ISSN 1040-4651
Preview |
PDF (Kourelis_etal_2024_ThePlantCell_koae183)
- Published Version
Available under License Creative Commons Attribution. Download (2MB) | Preview |
Abstract
Secreted immune proteases “Required for Cladosporium resistance-3” (Rcr3) and “Phytophthora-inhibited protease-1” (Pip1) of tomato (Solanum lycopersicum) are both inhibited by Avirulence-2 (Avr2) from the fungal plant pathogen Cladosporium fulvum. However, only Rcr3 acts as a decoy co-receptor that detects Avr2 in the presence of the Cf-2 immune receptor. Here, we identified crucial residues in tomato Rcr3 that are required for Cf-2-mediated signaling and bioengineered various proteases to trigger Avr2/Cf-2-dependent immunity. Despite substantial divergence in Rcr3 orthologs from eggplant (Solanum melongena) and tobacco (Nicotiana spp.), minimal alterations were sufficient to trigger Avr2/Cf-2-mediated immune signaling. By contrast, tomato Pip1 was bioengineered with 16 Rcr3-specific residues to initiate Avr2/Cf-2-triggered immune signaling. These residues cluster on one side of the protein next to the substrate-binding groove, indicating a potential Cf-2 interaction site. Our findings also revealed that Rcr3 and Pip1 have distinct substrate preferences determined by two variant residues and that both are suboptimal for binding Avr2. This study advances our understanding of Avr2 perception and opens avenues to bioengineer proteases to broaden pathogen recognition in other crops.
| Item Type: | Article |
|---|---|
| Additional Information: | Data availability statement: Uncropped gels are provided in Supplementary File 1. Used plasmids are listed in Supplementary Data Set 2, and plasmid maps are provided in Supplementary File 2. Funding Information: This work was supported by “The Clarendon Fund” (J.K.) and the European Research Council ERC-CoG-2013 grant 616449 “GreenProteases” (R.H., J.K.); European Research Council ERC-AdG-2020 grant 101019324 “ExtraImmune” (B.M. and R.H.); and BBSRC 18RM1 grant BB/S003193/1 “Pip1S” (M.S. and R.H.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
| Uncontrolled Keywords: | plant science ,/dk/atira/pure/subjectarea/asjc/1100/1110 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Science > The Sainsbury Laboratory |
| UEA Research Groups: | Faculty of Science > Research Groups > Plant Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 23 Oct 2024 10:30 |
| Last Modified: | 31 Oct 2024 00:52 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97136 |
| DOI: | 10.1093/plcell/koae183 |
Downloads
Downloads per month over past year
Actions (login required)
 |
View Item |