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King, Julie, Grewal, Surbhi, Othmeni, Manel, Coombes, Benedict, Yang, Cai-yun, Walter, Nicola, Ashling, Stephen, Scholefield, Duncan, Walker, Jack, Hubbart-Edwards, Stella, Hall, Anthony 
ORCID: https://orcid.org/0000-0002-1806-020X and King, Ian Phillip
(2022)
Introgression of the Triticum timopheevii genome into wheat detected by chromosome-specific Kompetitive allele specific PCR markers.
Frontiers in Plant Science, 13.
ISSN 1664-462X
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Abstract
Triticum timopheevii (2n = 28, AtAtGG) is a tetraploid wild relative species with great potential to increase the genetic diversity of hexaploid wheat Triticum aestivum (2n = 42, AABBDD) for various important agronomic traits. A breeding scheme that propagated advanced backcrossed populations of wheat-T. timopheevii introgression lines through further backcrossing and self-fertilisation resulted in the generation of 99 introgression lines (ILs) that carried 309 homozygous segments from the At and G subgenomes of T. timopheevii. These introgressions contained 89 and 74 unique segments from the At and G subgenomes, respectively. These overlapping segments covered 98.9% of the T. timopheevii genome that has now been introgressed into bread wheat cv. Paragon including the entirety of all T. timopheevii chromosomes via varying sized segments except for chromosomes 3At, 4G, and 6G. Homozygous ILs contained between one and eight of these introgressions with an average of three per introgression line. These homozygous introgressions were detected through the development of a set of 480 chromosome-specific Kompetitive allele specific PCR (KASP) markers that are well-distributed across the wheat genome. Of these, 149 were developed in this study based on single nucleotide polymorphisms (SNPs) discovered through whole genome sequencing of T. timopheevii. A majority of these KASP markers were also found to be T. timopheevii subgenome specific with 182 detecting At subgenome and 275 detecting G subgenome segments. These markers showed that 98% of the At segments had recombined with the A genome of wheat and 74% of the G genome segments had recombined with the B genome of wheat with the rest recombining with the D genome of wheat. These results were validated through multi-colour in situ hybridisation analysis. Together these homozygous wheat-T. timopheevii ILs and chromosome-specific KASP markers provide an invaluable resource to wheat breeders for trait discovery to combat biotic and abiotic stress factors affecting wheat production due to climate change.
| Item Type: | Article |
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| Additional Information: | Data Availability Statement: Raw reads data for T. timopheevii has been made available through the Grassroots data repository hosted by the Earlham Institute and funded by DFW programme (https://opendata.earlham.ac.uk/wheat). The genotyping data is available from the corresponding authors on reasonable request. Funding Information: This work was supported by the Biotechnology and Biological Sciences Research Council (grant numbers BB/J004596/1 and BB/P016855/1) as part of the Wheat Improvement Strategic Programme (WISP) and Developing Future Wheat (DFW) programme. MO and JW were supported by the Biotechnology and Biological Sciences Research Council (grant number BB/S012796/1) as part of the International Wheat Yield Partnership (IWYP) programme. BC was supported by the BBSRC funded Norwich Research Park Biosciences Doctoral Training Partnership grant BB/M011216/1 and NW was supported by BBSRC funded Nottingham Doctoral Training Partnership grant BB/T008369/1. |
| Uncontrolled Keywords: | gish,kasp markers,triticum timopheevii,wheat,wild relative introgression,plant science,sdg 13 - climate action ,/dk/atira/pure/subjectarea/asjc/1100/1110 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 23 Oct 2024 09:30 |
| Last Modified: | 28 Oct 2024 00:53 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97126 |
| DOI: | 10.3389/fpls.2022.919519 |
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