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ToolsKaufmann, Moritz, Li, Leilei, Van Poucke, Christof, Rhyner, Nicola, De Tender, Caroline, Uyttendaele, Mieke, Heyndrickx, Marc, Zipfel, Cyril, Pothier, Joël F. and Cottyn, Bart (2024) Soil type and associated microbiome influence chitin's growth promotion effect in lettuce. Phytobiomes Journal, 8 (3). pp. 248-261. ISSN 2471-2906
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Abstract
Chitin amendment of peat substrate has been proven effective in promoting lettuce growth and increasing phenolic compounds in lettuce seedlings. However, the effect of chitin soil amendment on lettuce growth in mineral soil remains unexplored. The effect of chitin amendment of mineral soil on lettuce growth and metabolite changes was investigated for the first time in the present study in comparison with chitin-amended peat substrate. Our findings showed that chitin addition in peat substrate increased lettuce head weight by approximately 50% at harvest, whereas this increase was 30% when chitin was added to mineral soil. Targeted metabolomics analysis indicated that chitin addition affected the phenolic compounds in lettuce seedlings, but this effect varied between soil types. Moreover, untargeted metabolomics analysis suggested that using peat substrate or mineral soil had a greater influence on produced lettuce metabolites than chitin addition. Rhizobiome analysis showed that specifically Mortierellaceae family members, known for chitin degradation and plant growth promotion, significantly increased in peat substrate upon chitin treatment. In mineral soil, three bacterial genera and five fungi, including known plant-growth-promoting genera, were significantly more abundant upon chitin treatment but Mortierellaceae family members were not. We assume that the observed effects primarily stem from soil characteristics and from chitin-induced alterations in rhizobiome composition, particularly the presence of Mortierellaceae members, leading to promoted lettuce growth. Despite the variability, chitin remains an environmentally friendly alternative to synthetic fertilizers in lettuce production, but its beneficial effects are dependent on rhizobiome composition, which should be considered before chitin application.
| Item Type: | Article |
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| Additional Information: | Data availability statement: Sequence data are available in the ENA short read archive under the accession PRJEB70956. Acknowledgements: Support was provided in Switzerland by the Swiss National Science Foundation (SNSF; grant 189340) and in Belgium by the Fonds Wetenschappelijk Onderzoek\u2013Vlaanderen (FWO; grant G063820N). C. Zipfel was supported by the University of Zurich. J. F. Pothier was supported by the Department of Life Sciences and Facility Management of the Zurich University of Applied Sciences in W\u00E4denswil. The open access article processing charges of this publication were funded by the Swiss National Science Foundation. We thank the greenhouse team of ILVO, Stijn Degroote (ILVO), Chris Van Vaes (ILVO), and Cecilia Lai (ZHAW) for their assistance in greenhouse and lab work, as well as the HPC team of the ZHAW for providing computational services. |
| Uncontrolled Keywords: | chitin,growth promotion,lettuce,metabarcoding,metabolomics,ecology, evolution, behavior and systematics,ecology,molecular biology,agronomy and crop science,plant science ,/dk/atira/pure/subjectarea/asjc/1100/1105 |
| Faculty \ School: | Faculty of Science > The Sainsbury Laboratory |
| UEA Research Groups: | Faculty of Science > Research Groups > Plant Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 23 Oct 2024 09:30 |
| Last Modified: | 01 Nov 2024 13:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97124 |
| DOI: | 10.1094/PBIOMES-12-23-0132-R |
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