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Gooch, Harriet C. C., Labedan, Marjorie, Hall, Lindsay J. 
ORCID: https://orcid.org/0000-0001-8938-5709 and Maxwell, Anthony
(2024)
Transplanting human infant gut microbiome species into Galleria mellonella.
BMC Research Notes, 17.
ISSN 1756-0500
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Abstract
Objective: Study of the human infant gut microbiome requires the use of surrogate mammalian species such as mice. We sought to investigate the usefulness of the greater wax moth larva, Galleria mellonella, as an alternative. Results: We have analysed the native gut microbiome of Galleria and developed methods for clearing the native microbiome and introducing species from human infant faecal samples. We find that some species, e.g. enterococci, are more successful at recolonisation, but that others, e.g. Bifidobacterium, are less so. The work paves the way for using Galleria rather than mice in this and similar work.
| Item Type: | Article |
|---|---|
| Additional Information: | Data availability statement: All data/materials can be obtained by contacting the senior author (tony.maxwell@jic.ac.uk). Sequencing data have been submitted to European Nucleotide Archive (ENA) database, accession number: ERP157621 (https://www.ebi.ac.uk/ena/browser/view/PRJEB72824). Funding information: NC3Rs (NC/R001782/1) and a Biotechnology and Biosciences Research Council (UK) Institute Strategic Programme Grant (BB/P012523/1). |
| Uncontrolled Keywords: | bifidobacterium,enterococcus,galleria,microbiome,biochemistry, genetics and molecular biology(all) ,/dk/atira/pure/subjectarea/asjc/1300 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 23 Oct 2024 09:30 |
| Last Modified: | 23 Oct 2024 09:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97119 |
| DOI: | 10.1186/s13104-024-06785-w |
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