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Pingel, S., Field, R. A. 
ORCID: https://orcid.org/0000-0001-8574-0275, Guther, M. L. S., Duszenko, M. and Ferguson, M. A. J.
(1995)
The hydrophobic mannoside Manα1-6Manα1-S-(CH2)7-CH3 acts as an acceptor for the UDP-Gal:glycosylphosphatidylinositol anchor α1,3-galactosyltransferase of Trypanosoma brucei.
Biochemical Journal, 309 (3).
pp. 877-882.
ISSN 0264-6021
Abstract
The variant surface glycoproteins (VSGs) of Trypanosoma brucei are attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) membrane anchor, This anchor contains the core sequence ethanolamine-PO4-6Manα1-2Manα1-6Manα1-4GlcNα1-6myo- inositol, which is conserved in all GPI anchors, and a unique αGal side chain attached to the 3-position of the αMan residue adjacent to the αGlcN residue. Here we report that trypanosome membranes can catalyse the transfer of Gal from UDP-Gal to the hydrophobic thioglycoside Manα1-6Manα1-S-(CH2)7-CH2. Characterization of the galactosylated products by electrospray mass spectrometry, exoglycosidase digestion and periodate-oxidation studies revealed that the major product was Manα1-6(Galα1-3)Manα1-S-(CH2)7-CH2. The similarity of this product to part of the mature VSG GPI anchor suggests that the thioglycoside is able to act as an acceptor for the trypanosome-specific UDP-Gal-GPI anchor α1,3-galactosyltransferase.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | biochemistry,molecular biology,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1303 |
| Faculty \ School: | Faculty of Science > School of Chemistry, Pharmacy and Pharmacology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 11 Sep 2024 14:30 |
| Last Modified: | 25 Sep 2024 18:09 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/96717 |
| DOI: | 10.1042/bj3090877 |
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