Detection of enzyme-catalyzed polysaccharide synthesis on surfaces

Clé, Carla, Martin, Cathie, Field, Robert A. ORCID: https://orcid.org/0000-0001-8574-0275, Kuzmič, Petr and Bornemann, Stephen (2010) Detection of enzyme-catalyzed polysaccharide synthesis on surfaces. Biocatalysis and Biotransformation, 28 (1). pp. 64-71. ISSN 1024-2422

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Abstract

Strategically important cellular components, such as the cell wall and the starch granule, present surfaces during their biosynthesis and degradation. The enzymology of such surfaces is experimentally challenging and goes well beyond classical solution-state analyses. The kinetics of surface catalysis is complex but tractable. A number of approaches to monitor surface catalysis are reviewed and each is suited to a different biological problem. Particular attention is paid to a method we have recently developed for quantitatively monitoring polysaccharide synthesis on a surface in real time using surface plasmon resonance spectroscopy. This method has many attractive features with the potential to tackle both biological and industrial problems.

Item Type:	Article
Additional Information:	Funding Information: We acknowledge the Marie Curie Early Stage Training grant MEST-CT-2004-504273 for the funding of C.C. and the Biotechnology and Biological Sciences Research Council for the core funding of the John Innes Centre. We thank Nikolaus Wellner for the FTIR microscopy.
Uncontrolled Keywords:	catalysis,enzyme,kinetics,polysaccharide,surface,surface plasmon resonance spectroscopy,biotechnology,catalysis,biochemistry ,/dk/atira/pure/subjectarea/asjc/1300/1305
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Science > School of Chemistry, Pharmacy and Pharmacology Faculty of Science > School of Chemistry (former - to 2024)
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	06 Sep 2024 13:34
Last Modified:	25 Sep 2024 18:07
URI:	https://ueaeprints.uea.ac.uk/id/eprint/96593
DOI:	10.3109/10242420903388744

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