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Møller, Svenning Rune, Yi, Xueying, Velásquez, Silvia Melina, Gille, Sascha, Hansen, Pernille Louise Munke, Poulsen, Christian P., Olsen, Carl Erik, Rejzek, Martin, Parsons, Harriet, Zhang, Yang, Wandall, Hans H., Clausen, Henrik, Field, Robert A. 
ORCID: https://orcid.org/0000-0001-8574-0275, Pauly, Markus, Estevez, Jose M., Harholt, Jesper, Ulvskov, Peter and Petersen, Bent Larsen
(2017)
Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD.
Scientific Reports, 7.
ISSN 2045-2322
Abstract
Extensins are plant cell wall glycoproteins that act as scaffolds for the deposition of the main wall carbohydrate polymers, which are interlocked into the supramolecular wall structure through intra- and inter-molecular iso-di-tyrosine crosslinks within the extensin backbone. In the conserved canonical extensin repeat, Ser-Hyp 4, serine and the consecutive C4-hydroxyprolines (Hyps) are substituted with an α-galactose and 1-5 β- or α-linked arabinofuranoses (Arafs), respectively. These modifications are required for correct extended structure and function of the extensin network. Here, we identified a single Arabidopsis thaliana gene, At3g57630, in clade E of the inverting Glycosyltransferase family GT47 as a candidate for the transfer of Araf to Hyp-arabinofuranotriose (Hyp-β1,4Araf-β1,2Araf-β1,2Araf) side chains in an α-linkage, to yield Hyp-Araf 4 which is exclusively found in extensins. T-DNA knock-out mutants of At3g57630 showed a truncated root hair phenotype, as seen for mutants of all hitherto characterized extensin glycosylation enzymes; both root hair and glycan phenotypes were restored upon reintroduction of At3g57630. At3g57630 was named Extensin Arabinose Deficient transferase, ExAD, accordingly. The occurrence of ExAD orthologs within the Viridiplantae along with its' product, Hyp-Araf 4, point to ExAD being an evolutionary hallmark of terrestrial plants and charophyte green algae.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: This work was supported by The Danish Councils for Strategic and Independent Research (12-125709, 12- 131859), The Danish National Research Foundation (DNRF107), The Innovation Fund Denmark (contract 5152- 0000), The Carlsberg Foundation DK, The Villum Foundation's Young Investigator Program DK, The Copenhagen University Excellence Program for Interdisciplinary Research (CDO2016), The EU Marie Sklodowska-Curie program (ExHoMo, PIEF-GA-2011-301401), The Deutsche Forschungsgemeinschaft grant (EXC 1028), The UK BBSRC Institute Strategic Programme (MET, BB/J004561/1), The UK John Innes Foundation and The ANPCyTArgentina (PICT2013-003, PICT2014-0504) and ICGEB (CRP/ARG16-03) programmes Publisher Copyright: © The Author(s) 2017. |
| Uncontrolled Keywords: | general ,/dk/atira/pure/subjectarea/asjc/1000 |
| Faculty \ School: | Faculty of Science > School of Chemistry, Pharmacy and Pharmacology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 04 Sep 2024 12:33 |
| Last Modified: | 25 Sep 2024 18:06 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/96519 |
| DOI: | 10.1038/srep45341 |
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