Complex pectin metabolism by gut bacteria reveals novel catalytic functions

Ndeh, Didier, Rogowski, Artur, Cartmell, Alan, Luis, Ana S., Baslé, Arnaud, Gray, Joseph, Venditto, Immacolata, Briggs, Jonathon, Zhang, Xiaoyang, Labourel, Aurore, Terrapon, Nicolas, Buffetto, Fanny, Nepogodiev, Sergey, Xiao, Yao, Field, Robert A. ORCID: https://orcid.org/0000-0001-8574-0275, Zhu, Yanping, O'Neill, Malcolm A., Urbanowicz, Breeanna R., York, William S., Davies, Gideon J., Abbott, D. Wade, Ralet, Marie Christine, Martens, Eric C., Henrissat, Bernard and Gilbert, Harry J. (2017) Complex pectin metabolism by gut bacteria reveals novel catalytic functions. Nature, 544 (7648). pp. 65-70. ISSN 0028-0836

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Abstract

The metabolism of carbohydrate polymers drives microbial diversity in the human gut microbiota. It is unclear, however, whether bacterial consortia or single organisms are required to depolymerize highly complex glycans. Here we show that the gut bacterium Bacteroides thetaiotaomicron uses the most structurally complex glycan known: The plant pectic polysaccharide rhamnogalacturonan-II, cleaving all but 1 of its 21 distinct glycosidic linkages. The deconstruction of rhamnogalacturonan-II side chains and backbone are coordinated to overcome steric constraints, and the degradation involves previously undiscovered enzyme families and catalytic activities. The degradation system informs revision of the current structural model of rhamnogalacturonan-II and highlights how individual gut bacteria orchestrate manifold enzymes to metabolize the most challenging glycan in the human diet.

Item Type:	Article
Additional Information:	Publisher Copyright: © 2017 Macmillan Publishers Limited, part of Springer Nature. All rights reserved.
Uncontrolled Keywords:	general ,/dk/atira/pure/subjectarea/asjc/1000
Faculty \ School:	Faculty of Science > School of Chemistry, Pharmacy and Pharmacology
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	04 Sep 2024 12:33
Last Modified:	25 Sep 2024 18:06
URI:	https://ueaeprints.uea.ac.uk/id/eprint/96518
DOI:	10.1038/nature21725

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