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O'Neill, Ellis C., Pergolizzi, Giulia, Stevenson, Clare E.M., Lawson, David M., Nepogodiev, Sergey A. and Field, Robert A. 
ORCID: https://orcid.org/0000-0001-8574-0275
(2017)
Cellodextrin phosphorylase from Ruminiclostridium thermocellum:X-ray crystal structure and substrate specificity analysis.
Carbohydrate Research, 451.
pp. 118-132.
ISSN 0008-6215
Abstract
The GH94 glycoside hydrolase cellodextrin phosphorylase (CDP, EC 2.4.1.49) produces cellodextrin oligomers from short β-1→4-glucans and α-D-glucose 1-phosphate. Compared to cellobiose phosphorylase (CBP), which produces cellobiose from glucose and α-D-glucose 1-phosphate, CDP is biochemically less well characterised. Herein, we investigate the donor and acceptor substrate specificity of recombinant CDP from Ruminiclostridium thermocellum and we isolate and characterise a glucosamine addition product to the cellobiose acceptor with the non-natural donor α-D-glucosamine 1-phosphate. In addition, we report the first X-ray crystal structure of CDP, along with comparison to the available structures from CBPs and other closely related enzymes, which contributes to understanding of the key structural features necessary to discriminate between monosaccharide (CBP) and oligosaccharide (CDP) acceptor substrates.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: These studies were supported by the UK BBSRC Institute Strategic Program on Understanding and Exploiting Metabolism (MET) [BB/J004561/1] and the John Innes Foundation; the BBSRC, EPSRC and InnovateUK: IBCatalyst [BB/M02903411]. We acknowledge the Diamond Light Source for access to beamline I02 under proposal MX7641. Funding Information: These studies were supported by the UK BBSRC Institute Strategic Program on Understanding and Exploiting Metabolism (MET) [ BB/J004561/1 ] and the John Innes Foundation ; the BBSRC, EPSRC and InnovateUK: IBCatalyst [ BB/M02903411 ]. We acknowledge the Diamond Light Source for access to beamline I02 under proposal MX7641. Publisher Copyright: © 2017 The Authors |
| Uncontrolled Keywords: | cellodextrin phosphorylase,glucosamine 1-phosphate,x-ray crystal structure,analytical chemistry,biochemistry,organic chemistry ,/dk/atira/pure/subjectarea/asjc/1600/1602 |
| Faculty \ School: | Faculty of Science > School of Chemistry, Pharmacy and Pharmacology Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 04 Sep 2024 09:35 |
| Last Modified: | 25 Sep 2024 18:06 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/96513 |
| DOI: | 10.1016/j.carres.2017.07.005 |
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