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Wangpaiboon, Karan, Padungros, Panuwat, Nakapong, Santhana, Charoenwongpaiboon, Thanapon, Rejzek, Martin, Field, Robert A. 
ORCID: https://orcid.org/0000-0001-8574-0275 and Pichyangkura, Rath
(2018)
An α-1,6-and α-1,3-linked glucan produced by Leuconostoc citreum ABK-1 alternansucrase with nanoparticle and film-forming properties.
Scientific Reports, 8 (1).
ISSN 2045-2322
Abstract
Alternansucrase catalyses the sequential transfer of glucose residues from sucrose onto another sucrose molecule to form a long chain polymer, known as "alternan". The alternansucrase-encoding gene from Leuconostoc citreum ABK-1 (Lcalt) was successfully cloned and expressed in Escherichia coli. Lcalt encoded LcALT of 2,057 amino acid residues; the enzyme possessed an optimum temperature and pH of 40 °C and 5.0, respectively, and its' activity was stimulated up to 2.4-fold by the presence of Mn2+. Kinetic studies of LcALT showed a high transglycosylation activity, with Km 32.2 ± 3.2 mM and kcat 290 ± 12 s-1. Alternan generated by LcALT (Lc-alternan) harbours partially alternating α-1,6 and α- 1,3 glycosidic linkages confirmed by NMR spectroscopy, methylation analysis, and partial hydrolysis of Lc-alternan products. In contrast to previously reported alternans, Lc-alternan can undergo self-assembly, forming nanoparticles with an average size of 90 nm in solution. At concentrations above 15% (w/v), Lc-alternan nanoparticles disassemble and form a high viscosity solution, while this polymer forms a transparent film once dried.
| Item Type: | Article |
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| Additional Information: | Funding Information: This work was funded by “Chulalongkorn University Graduate School scholarship, commemorating the 72nd anniversary of his Majesty King Bhumibol Aduladej” and the 90th Anniversary of Ratchadaphiseksomphot Endowment Fund of Chulalongkorn University (Grant GCUGR1125603018D). We greatly thank Dr. Pawinee Panpetch and the Starch and Cyclodextrin Research Unit, Department of Biochemistry, Faculty of Science, Chulalongkorn University for proofreading and their support on HPAEC-PAD analysis. Prof. Dr. Suwabun Chirachanchai, Petroleum and Petrochemical College, Chulalongkorn University, for the Zetasizer Nano ZS analysis. Dr. Michael D. Rugen and Dr. Paul Brett, Department of Biological Chemistry, John Innes Centre, Norwich Research Park for proofreading and assistance with GC-MS. Publisher Copyright: © 2018 The Author(s). |
| Uncontrolled Keywords: | general ,/dk/atira/pure/subjectarea/asjc/1000 |
| Faculty \ School: | Faculty of Science > School of Chemistry, Pharmacy and Pharmacology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 04 Sep 2024 08:33 |
| Last Modified: | 25 Sep 2024 18:06 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/96502 |
| DOI: | 10.1038/s41598-018-26721-w |
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