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Ahmadipour, Sanaz, Winsbury, Rebecca, Köhler, Dominic, Pergolizzi, Giulia, Nepogodiev, Sergey A., Chessa, Simona, Dedola, Simone, Wang, Meng, Voglmeir, Josef and Field, Robert A. 
ORCID: https://orcid.org/0000-0001-8574-0275
(2023)
β-1,2-Oligomannan phosphorylase-mediated synthesis of potential oligosaccharide vaccine candidates.
Carbohydrate Research, 528.
ISSN 0008-6215
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Abstract
β-(1,2)-Mannan antigens incorporated into vaccines candidates for immunization studies, showed that antibodies raised against β-(1,2)-mannotriose antigens can protect against disseminated candidiasis. Until recently, β-(1,2)- mannans could only be obtained by isolation from microbial cultures, or by lengthy synthetic strategies involving protecting group manipulation. The discovery of two β-(1,2)-mannoside phosphorylases, Teth514_1788 and Teth514_1789, allowed efficient access to these compounds. In this work, Teth514_1788 was utilised to generate β-(1,2)-mannan antigens, tri- and tetra-saccharides, decorated with a conjugation tether at the reducing end, suitable to be incorporated on a carrier en-route to novel vaccine candidates, illustrated here by conjugation of the trisaccharide to BSA.
| Item Type: | Article |
|---|---|
| Additional Information: | Data availability statement: No data was used for the research described in the article. Funding Information: Innovate UK in the form of an Innovation Scholar Secondment to SA (UKRI, 76242: Sugars, Enzymes and Diagnostics (SEAD)). Work at the John Innes Centre was supported by the UK BBSRC Institute Strategic Program on Molecules from Nature -Products and Pathways [ BBS/E/J/000PR9790 ] and the John Innes Foundation. |
| Uncontrolled Keywords: | candida pathogen,click chemistry,glycoconjugate vaccines,β-(1,2)-mannan antigen,β-(1,2)-oligomannan phosphorylase,analytical chemistry,biochemistry,organic chemistry,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/1600/1602 |
| Faculty \ School: | Faculty of Science > School of Chemistry, Pharmacy and Pharmacology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 03 Sep 2024 15:35 |
| Last Modified: | 02 Dec 2024 01:44 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/96480 |
| DOI: | 10.1016/j.carres.2023.108807 |
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