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Charoenwongpaiboon, Thanapon, Wangpaiboon, Karan, Pichyangkura, Rath, Nepogodiev, Sergey A., Wonganan, Piyanuch, Mahalapbutr, Panupong and Field, Robert A. 
ORCID: https://orcid.org/0000-0001-8574-0275
(2021)
Characterization of a nanoparticulate exopolysaccharide from Leuconostoc holzapfelii KM01 and its potential application in drug encapsulation.
International Journal of Biological Macromolecules, 187.
pp. 690-698.
ISSN 0141-8130
Abstract
Fermentation of Lactic Acid Bacteria (LAB) is considered to be a sustainable approach for polysaccharide production. Herein, exopolysaccharide (EPS)-producing LAB strain KM01 was isolated from Thai fermented dessert, Khao Mak, which was then identified as Leuconostoc holzapfelii. High-performance anion-exchange chromatography, nuclear magnetic resonance spectroscopy and Fourier-transform infrared spectroscopy suggested that the KM01 EPS comprises α-1,6-linked glucosides. The molecular weight of KM01 EPS was around 500 kDa, but it can form large aggregates formation (MW > 2000 kDa) in an aqueous solution, judged by transmission electron microscopy and dynamic light scattering to be around 150 nm in size. Furthermore, this KM01 EPS form highly viscous hydrogels at concentrations above 5% (w/v). The formation of hydrogels and nanoparticle of KM01 EPS was found to be reversible. Finally, the suitability of KM01 EPS for biomedical applications was demonstrated by its lack of cytotoxicity and its ability to form complexes with quercetin. Unlike the common α-1,6-linked dextran, KM01 EPS can enhance the solubility of quercetin significantly.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: We would like to thank Department of Biochemistry, Faculty of Science, Chulalongkorn University; Department of Chemistry, Faculty of Science, Silpakorn University; and Department of Biological Chemistry, John Innes Centre, UK for all resources and facilities. Special thanks to Prof. Dr. Suwabun Chirachanchai for NMR resources. Publisher Copyright: © 2021 |
| Uncontrolled Keywords: | leuconostoc holzapfelii,nanoparticle,α-glucan,structural biology,biochemistry,molecular biology ,/dk/atira/pure/subjectarea/asjc/1300/1315 |
| Faculty \ School: | Faculty of Science > School of Chemistry, Pharmacy and Pharmacology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 03 Sep 2024 09:37 |
| Last Modified: | 25 Sep 2024 18:05 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/96466 |
| DOI: | 10.1016/j.ijbiomac.2021.07.174 |
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