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Baker, Alexander N., Richards, Sarah-Jane, Pandey, Sarojini, Guy, Collette S., Ahmad, Ashfaq, Hasan, Muhammad, Biggs, Caroline I., Georgiou, Panagiotis G., Zwetsloot, Alexander J., Straube, Anne, Dedola, Simone, Field, Robert A. 
ORCID: https://orcid.org/0000-0001-8574-0275, Anderson, Neil R., Walker, Marc, Grammatopoulos, Dimitris and Gibson, Matthew I.
(2021)
Glycan-based flow-through device for the detection of SARS-COV-2.
ACS Sensors, 6 (10).
pp. 3696-3705.
ISSN 2379-3694
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Abstract
The COVID-19 pandemic, and future pandemics, require diagnostic tools to track disease spread and guide the isolation of (a)symptomatic individuals. Lateral-flow diagnostics (LFDs) are rapid and of lower cost than molecular (genetic) tests, with current LFDs using antibodies as their recognition units. Herein, we develop a prototype flow-through device (related, but distinct to LFDs), utilizing N-acetyl neuraminic acid-functionalized, polymer-coated, gold nanoparticles as the detection/capture unit for SARS-COV-2, by targeting the sialic acid-binding site of the spike protein. The prototype device can give rapid results, with higher viral loads being faster than lower viral loads. The prototype's effectiveness is demonstrated using spike protein, lentiviral models, and a panel of heat-inactivated primary patient nasal swabs. The device was also shown to retain detection capability toward recombinant spike proteins from several variants (mutants) of concern. This study provides the proof of principle that glyco-lateral-flow devices could be developed to be used in the tracking monitoring of infectious agents, to complement, or as alternatives to antibody-based systems.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: M.I.G. is supported by the Royal Society (Industry Fellowship 191037) and the ERC (866056). The BBSRC MIBTP program (BB/M01116X/1) and Iceni Diagnostics Ltd. are thanked for supporting A.N.B. BBSRC/InnovateUK are thanked for funding the Specialty Glycans project BB/M02878X/1. UoW, EPSRC (EP/R511808/1), and BBSRC (BB/S506783/1) impact acceleration accounts are thanked, as is UoW for funding. The Warwick Polymer Research Technology Platforms is acknowledged for SEC analysis. This project has received funding from the European Union’s Horizon 2020 Research and Innovation Programme under the Marie Skłodowska–Curie Grant Agreement No. 814236. The Leverhulme Trust is thanked for support (RPG-2019-087). A.J.Z. is funded by the MRC DTP (MR/N014294/1). A.S. is a Wellcome Trust Investigator 200870/Z/16/Z. Dr. Marta Neves, Dr. Kathryn Murray, and Angela Hurst are thanked for assisting in preparing the flow devices. The authors sincerely thank the technical and administrative staff of the UoW who enabled the author’s laboratories to remain open during the COVID-19 pandemic. Reagents were obtained through BEI Resources for evaluation of influenza binding. A complete list is in the Supporting Information. |
| Uncontrolled Keywords: | covid-19,flow-through,glycans,glycobiology,lateral flow,nanoparticles,polymers,rapid diagnostics,sars-cov-2,bioengineering,instrumentation,process chemistry and technology,fluid flow and transfer processes ,/dk/atira/pure/subjectarea/asjc/1500/1502 |
| Faculty \ School: | Faculty of Science > School of Chemistry, Pharmacy and Pharmacology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 03 Sep 2024 09:37 |
| Last Modified: | 11 Oct 2024 00:14 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/96465 |
| DOI: | 10.1021/acssensors.1c01470 |
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