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Baker, Alexander N., Muguruza, Asier R., Richards, Sarah Jane, Georgiou, Panagiotis G., Goetz, Stephen, Walker, Marc, Dedola, Simone, Field, Robert A. 
ORCID: https://orcid.org/0000-0001-8574-0275 and Gibson, Matthew I.
(2022)
Lateral flow glyco-assays for the rapid and low-cost detection of lectins–polymeric linkers and particle engineering are essential for selectivity and performance.
Advanced Healthcare Materials, 11 (4).
ISSN 2192-2640
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Abstract
Lateral flow immuno-assays, such as the home pregnancy test, are rapid point-of-care diagnostics that use antibody-coated nanoparticles to bind antigens/analytes (e.g., viruses, toxins or hormones). Ease of use, no need for centralized infrastructure and low-cost, makes these devices appealing for rapid disease identification, especially in low-resource environments. Here glycosylated polymer-coated nanoparticles are demonstrated for the sensitive, label-free detection of lectins in lateral flow and flow-through. The systems introduced here use glycans, not antibodies, to provide recognition: a “lateral flow glyco-assay,” providing unique biosensing opportunities. Glycans are installed onto polymer termini and immobilized onto gold nanoparticles, providing colloidal stability but crucially also introducing assay tunability and selectivity. Using soybean agglutinin and Ricinus communis agglutinin I (RCA120) as model analytes, the impact of polymer chain length and nanoparticle core size are evaluated, with chain length found to have a significant effect on signal generation—highlighting the need to control the macromolecular architecture to tune response. With optimized systems, lectins are detectable at subnanomolar concentrations, comparable to antibody-based systems. Complete lateral flow devices are also assembled to show how these devices can be deployed in the “real world.” This work shows that glycan-binding can be a valuable tool in rapid diagnostics.
| Item Type: | Article |
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| Additional Information: | Funding Information: M.I.G. was supported by the ERC (866056). The BBSRC‐funded MIBTP program (BB/M01116X/1) and Iceni Diagnostics Ltd. are thanked for a studentship for A.N.B. The BBSRC‐funded MIBTP program (BB/M01116X/1) is thanked for the studentship of A.R.M. This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska‐Curie grant agreement No. 814236 (P.G.G.). BBSRC/Innovate is thanked for funding the Specialty Glycans project BB/M02878X/1 (S.‐J.R.). The Warwick Polymer Research Technology Platform is acknowledged for SEC analysis. The Warwick Polymer and Electron Microscopy Research Technology Platforms (Y. Han) are acknowledged for the SEC/EM analysis. |
| Uncontrolled Keywords: | carbohydrates,diagnostics,glyco-assays,gold nanoparticles,lateral flow devices,lectins,polymers,biomaterials,biomedical engineering,pharmaceutical science ,/dk/atira/pure/subjectarea/asjc/2500/2502 |
| Faculty \ School: | Faculty of Science > School of Chemistry, Pharmacy and Pharmacology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 03 Sep 2024 09:36 |
| Last Modified: | 04 Dec 2024 01:41 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/96464 |
| DOI: | 10.1002/adhm.202101784 |
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