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Polsinelli, Ivan, Caliandro, Rosanna, Salomone-Stagni, Marco, Demitri, Nicola, Rejzek, Martin, Field, Robert A. 
ORCID: https://orcid.org/0000-0001-8574-0275 and Benini, Stefano
(2019)
Comparison of the Levansucrase from the epiphyte Erwinia tasmaniensis vs its homologue from the phytopathogen Erwinia amylovora.
International Journal of Biological Macromolecules, 127.
pp. 496-501.
ISSN 0141-8130
Abstract
Erwinia tasmaniensis is an epiphytic bacterium related to the plant pathogen Erwinia amylovora, the etiological agent of fire blight. In this study the levansucrase from E. tasmaniensis (EtLsc) has been compared with the homologous enzyme from E. amylovora (EaLsc). We characterized the enzymatic activity and compared the products profile of both enzymes by High Performance Anion Exchange Chromatography coupled with Pulsed Amperometric Detector (HPAEC-PAD). Moreover we determined the crystal structure of EtLsc to understand the structural peculiarity causing the different product profiles of the two homologues. EtLsc exhibits increased efficiency in the production of FOS, resulting in a better catalyst for biotechnological synthesis than EaLsc. Based on our results, we propose that the role of this enzyme in the life cycle of the two bacteria is most likely related to survival, rather than linked to pathogenicity in E. amylovora.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: The research leading to these results has received funding from the Free University of Bolzano under the MESCAL project (grant number 1440 ). IP was supported by a predoctoral fellowship from the Free University of Bolzano . We thank ELETTRA Sincrotrone Trieste for beamtime under proposal 20180463. The Plasmid pMCSG49 was obtained from the DNASU Plasmid Repository, Arizona State University under a signed Material Transfer Agreement. Work at the JIC was supported by the UK BBSRC Institute Strategic Program on Molecules from Nature (MfN) [ BB/PO12523/1 ] and the John Innes Foundation . Publisher Copyright: © 2019 Elsevier B.V. |
| Uncontrolled Keywords: | fire blight,fructooligosaccharides,fructosyltransferase,oligosaccharides,structural biology,biochemistry,molecular biology,economics and econometrics,energy(all) ,/dk/atira/pure/subjectarea/asjc/1300/1315 |
| Faculty \ School: | Faculty of Science > School of Chemistry, Pharmacy and Pharmacology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 02 Sep 2024 15:31 |
| Last Modified: | 25 Sep 2024 18:05 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/96454 |
| DOI: | 10.1016/j.ijbiomac.2019.01.074 |
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