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Charoenwongpaiboon, Thanapon, Pichyangkura, Rath, Field, Robert A. 
ORCID: https://orcid.org/0000-0001-8574-0275 and Prousoontorn, Manchumas Hengsakul
(2019)
Preparation of cross-linked enzyme aggregates (CLEAs) of an inulosucrase mutant for the enzymatic synthesis of inulin-type fructooligosaccharides.
Catalysts, 9 (8).
ISSN 2073-4344
Abstract
Fructooligosaccharides are well-known carbohydrate molecules that exhibit good probiotic activity and are widely used as sweeteners. Inulin-type fructooligosaccharides (IFOs) can be synthesized from sucrose using inulosucrase. In this study, cross-linked enzyme aggregates (CLEAs) of Lactobacillus reuteri 121 inulosucrase (R483A-LrInu) were prepared and used as a biocatalyst for IFOs production. Under optimum conditions, R483A-LrInu CLEAs retained 42% of original inulosucrase activity. Biochemical characterization demonstrated that the optimum pH of inulosucrase changed from 5 to 4 after immobilization, while the optimum temperature was unchanged. Furthermore, the pH stability and thermostability of the R483A-LrInu CLEAs was significantly improved. IFOs product characterization indicated that the product specificity of the enzyme was impacted by CLEA generation, producing a narrower range of IFOs than the soluble enzyme. In addition, the R483A-LrInu CLEAs showed operational stability in the batch synthesis of IFOs.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: This research was partially funded by the UK BBSRC Institute Strategic Program on Molecules from Nature (MfN) [BB/PO12523/1] and the John Innes Foundation. The APC was funded by John Innes Centre. Acknowledgments: TC is thankful for scholarship supports from Science Achievement Scholarship of Thailand (SAST), Faculty of Science Chulalongkorn University and Department of Biological Chemistry John Innes Centre. Funding Information: Funding: This research was partially funded by the UK BBSRC Institute Strategic Program on Molecules from Nature (MfN) [BB/PO12523/1] and the John Innes Foundation. The APC was funded by John Innes Centre. Publisher Copyright: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. |
| Uncontrolled Keywords: | cross-linked enzyme aggregates,fructooilgosaccharide,inulosucrase,catalysis,environmental science(all),physical and theoretical chemistry ,/dk/atira/pure/subjectarea/asjc/1500/1503 |
| Faculty \ School: | Faculty of Science > School of Chemistry, Pharmacy and Pharmacology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 02 Sep 2024 15:30 |
| Last Modified: | 25 Sep 2024 18:05 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/96442 |
| DOI: | 10.3390/catal9080641 |
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