Preparative and Kinetic Analysis of β-1,4- and β-1,3-Glucan Phosphorylases Informs Access to Human Milk Oligosaccharide Fragments and Analogues Thereof

Singh, Ravindra Pal, Pergolizzi, Giulia, Nepogodiev, Sergey A., de Andrade, Peterson, Kuhaudomlarp, Sakonwan and Field, Robert A. ORCID: https://orcid.org/0000-0001-8574-0275 (2020) Preparative and Kinetic Analysis of β-1,4- and β-1,3-Glucan Phosphorylases Informs Access to Human Milk Oligosaccharide Fragments and Analogues Thereof. ChemBioChem, 21 (7). pp. 1043-1049. ISSN 1439-4227

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Abstract

The enzymatic synthesis of oligosaccharides depends on the availability of suitable enzymes, which remains a limitation. Without recourse to enzyme engineering or evolution approaches, herein we demonstrate the ability of wild-type cellodextrin phosphorylase (CDP: β-1,4-glucan linkage-dependent) and laminaridextrin phosphorylase (Pro_7066: β-1,3-glucan linkage-dependent) to tolerate a number of sugar-1- phosphate substrates, albeit with reduced kinetic efficiency. In spite of catalytic efficiencies of <1 % of the natural reactions, we demonstrate the utility of given phosphorylase–sugar phosphate pairs to access new-to-nature fragments of human milk oligosaccharides, or analogues thereof, in multi-milligram quantities.

Item Type:	Article
Additional Information:	Funding Information: These studies were supported by the UK BBSRC Institute Strategic Program on Molecules from Nature—Products and Pathways [BBS/E/J/000PR9790] and the John Innes Foundation; the BBSRC and ESRC–Newton Fund [BB/N005082/1], the Department for International Development, and the Indian Department of Biotechnology under the Newton Fund Global Research Partnership in Aquaculture programme; and the InnovateUK IBCatalyst [BB/M02903411 and EP/N033167/10]. We thank Gerhard Saalbach for assistance with the mass spectrometry. Publisher Copyright: © 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.
Uncontrolled Keywords:	enzymatic synthesis,glycans,oligosaccharides,phosphorylases,biochemistry,molecular medicine,molecular biology,organic chemistry ,/dk/atira/pure/subjectarea/asjc/1300/1303
Faculty \ School:	Faculty of Science > School of Chemistry, Pharmacy and Pharmacology
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	02 Sep 2024 15:30
Last Modified:	25 Sep 2024 18:05
URI:	https://ueaeprints.uea.ac.uk/id/eprint/96436
DOI:	10.1002/cbic.201900440

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