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Wangpaiboon, Karan, Waiyaseesang, Nisachon, Panpetch, Pawinee, Charoenwongpaiboon, Thanapon, Nepogodiev, Sergey A., Ekgasit, Sanong, Field, Robert A. 
ORCID: https://orcid.org/0000-0001-8574-0275 and Pichayangkura, Rath
(2020)
Characterisation of insoluble α-1,3-/α-1,6 mixed linkage glucan produced in addition to soluble α-1,6-linked dextran by glucansucrase (DEX-N) from Leuconostoc citreum ABK-1.
International Journal of Biological Macromolecules, 152.
pp. 473-482.
ISSN 0141-8130
Abstract
Glucansucrases catalyse the formation of glucans from sucrose. The glucansucrase-encoding gene from Leuconostoc citreum ABK-1, dex-N, was successfully cloned and expressed in E. coli BL21 Star (DE3). DEX-N produces 2 types of glucans: soluble (S-dextran) and insoluble (I-glucan) glucans. The S-dextran was determined to be ca. 10 kDa in size and contained >90% α-1,6 linkages; along with its water solubility, this is similar to commercial dextran. On the other hand, I-glucan was water-insoluble, harbouring a block-wise pattern of α-1,3 and α-1,6 linkages in its structure. Notably, the FTIR and powder X-ray diffraction pattern of I-glucan exhibited a combination of features found in α-1,6-linked dextran and α-1,3-linked mutan. Although both I-glucan and mutan are insoluble glucans, their physical characteristics are notably dissimilar.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: This work was supported by a Postdoctoral fellowship from Chulalongkorn University Graduate School (KW). Research at the JIC is supported by the UK BBSRC Institute Strategic Program on Molecules from Nature (MfN) [ BB/PO12523/1 ] and the John Innes Foundation . We also thank Associated Prof. Dr. Kuakarun Krusong, Department of Biochemistry, Faculty of Science, Chulalongkorn University for standard dextrans; Prof. Dr. Siriporn Damrongsakkul, Department of Chemical engineering, Faculty of Engineering, Chulalongkorn University, for the Vibro viscometer SV-10; Dr. Giulia Pergolizzi and Dr. Irina Ivanova, Department of Biological Chemistry, John Innes Centre for chemistry advice; Dr. Paul Brett, Metabolite Analysis Platform, John Innes Centre for assisting with GC-MS analyses, and Ms. Gisma C. Asmarani, Department of Chemistry, Faculty of Science, Chulalongkorn University for assisting the FTIR analysis. Publisher Copyright: © 2020 |
| Uncontrolled Keywords: | block-wise glucan structure,glucan polymers,glucansucrase,glucosyltransferase-si,leuconostoc citreum,structural biology,biochemistry,molecular biology ,/dk/atira/pure/subjectarea/asjc/1300/1315 |
| Faculty \ School: | Faculty of Science > School of Chemistry, Pharmacy and Pharmacology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 02 Sep 2024 13:31 |
| Last Modified: | 25 Sep 2024 18:05 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/96430 |
| DOI: | 10.1016/j.ijbiomac.2020.02.247 |
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