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Baker, Alexander N., Richards, Sarah Jane, Guy, Collette S., Congdon, Thomas R., Hasan, Muhammad, Zwetsloot, Alexander J., Gallo, Angelo, Lewandowski, Józef R., Stansfeld, Phillip J., Straube, Anne, Walker, Marc, Chessa, Simona, Pergolizzi, Giulia, Dedola, Simone, Field, Robert A. 
ORCID: https://orcid.org/0000-0001-8574-0275 and Gibson, Matthew I.
(2020)
The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device.
ACS Central Science, 6 (11).
pp. 2046-2052.
ISSN 2374-7943
Abstract
There is an urgent need to understand the behavior of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: MIG is supported by the ERC (638661) and Royal Society (Industry Fellowship 191037). The BBSRC MIBTP program (BB/M01116X/1) and Iceni Diagnostics Ltd are thanked for support to A.N.B. UoW, EPSRC (EP/R511808/1), and BBSRC (BB/S506783/1) impact acceleration accounts are thanked. A.J.Z. is funded by the MRC DTP (MR/N014294/1). A.S. is a Wellcome Trust Investigator (200870/Z/16/Z). The Leverhulme Trust are thanked for support (RPG-2019-087). P.J.S. thanks Wellcome (208361/Z/17/Z), the MRC (MR/S009213/1), and BBSRC (BB/P01948X/1, BB/R002517/1, and BB/S003339/1). The Warwick Polymer and Electron Microscopy Research Technology Platforms (Y. Han) are acknowledged for the SEC/EM analysis. Dr C. Biggs is thanked for lateral flow device preparation. We sincerely thank the technical and administrative staff of the UoW who enabled our laboratory to remain open during the COVID-19 pandemic. Prof. Peter Scott is thanked for critically reading this manuscript. Publisher Copyright: © 2020 American Chemical Society. All rights reserved. |
| Uncontrolled Keywords: | chemistry(all),chemical engineering(all) ,/dk/atira/pure/subjectarea/asjc/1600 |
| Faculty \ School: | Faculty of Science > School of Chemistry, Pharmacy and Pharmacology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 02 Sep 2024 13:31 |
| Last Modified: | 25 Sep 2024 18:05 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/96428 |
| DOI: | 10.1021/acscentsci.0c00855 |
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